Western Blot (WB)
(NDST1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NDST1 antibody detected the NDST1 protein (arrow).)
Rabbit NDST1 Polyclonal Antibody | anti-NDST1 antibody
NDST1 Antibody (C-term)
Predicted Reactivity: Mouse, Rat
Predicted Reactivity: Mouse, Rat
IHC-P~~1:10~50
Western Blot (WB)
(NDST1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NDST1 antibody detected the NDST1 protein (arrow).)
Western Blot (WB)
(NDST1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NDST1 antibody detected the NDST1 protein (arrow).)
Immunohistochemistry (IHC)
(NDST1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDST1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(NDST1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDST1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NDST1 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugarbackbone to make N-sulfated heparosan, aprerequisite substrate for later modifications inheparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparansulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates inbiosynthesis of heparan sulfate that canultimately serve as L-selectin ligands, there by playing a role in inflammatory response
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Duelli, A., et al. J. Immunol. 183(11):7073-7083(2009)
Zuberi, R.I., et al. J. Immunol. 183(6):3971-3979(2009)
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Uniprot Description
NDST1: Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L- selectin ligands, thereby playing a role in inflammatory response. Belongs to the sulfotransferase 1 family. NDST subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Glycan Metabolism - heparan sulfate biosynthesis; Membrane protein, integral; Hydrolase; EC 2.8.2.8; Transferase
Chromosomal Location of Human Ortholog: 5q33.1
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: deacetylase activity; [heparan sulfate]-glucosamine N-sulfotransferase activity
Biological Process: smoothened signaling pathway; fibroblast growth factor receptor signaling pathway; glycosaminoglycan metabolic process; MAPKKK cascade; pathogenesis; embryonic viscerocranium morphogenesis; respiratory gaseous exchange; glycosaminoglycan biosynthetic process; polysaccharide biosynthetic process; heparin biosynthetic process; heparan sulfate proteoglycan biosynthetic process; carbohydrate metabolic process; forebrain development; midbrain development; embryonic neurocranium morphogenesis; inflammatory response
Disease: Mental Retardation, Autosomal Recessive 46
Research Articles on NDST1
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Product Notes
The NDST1 ndst1 (Catalog #AAA9214440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 820-849. The NDST1 Antibody (C-term) reacts with React: Human Predicted Reactivity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDST1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry Paraffin(IHC). WB~~1:1000 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the NDST1 ndst1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDST1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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