Rabbit anti-Human NDST1 Polyclonal Antibody | anti-NDST1 antibody

NDST1, CT (NDST1, HSST, HSST1, Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, N-heparan sulfate sulfotransferase 1, [Heparan sulfate]-glucosamine N-sulfotransferase 1, Heparan sulfate N-de

Cat. Num. AAA6324296

• Gene Names: NDST1; HSST; NST1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: NDST1; Polyclonal Antibody; CT (NDST1; HSST; HSST1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; Glucosaminyl N-deacetylase/N-sulfotransferase 1; N-heparan sulfate sulfotransferase 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; Heparan sulfate N-de; Heparan sulfate N-deacetylase 1; Heparan sulfate N-sulfotransferase 1; anti-NDST1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NDST1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-NDST1 antibody: Western Blot (WB), Immunohistochemistry (IHC), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 820-849 in the C-terminal region of human NDST1, UniProt Accession #P52848. Species sequence homology: mouse and rat
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS648643. This demonstrates that MBS648643 detected the NDST1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS648643 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-NDST1 antibody

NDST1 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.

Product Categories/Family for anti-NDST1 antibody

Antibodies; Enzymes; Sulfotransferase

References

1. Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). 2. Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). 3. Duelli, A., et al. J. Immunol. 183(11):7073-7083(2009). 4. Zuberi, R.I., et al. J. Immunol. 183(6):3971-3979(2009). 5. Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4505351
• NCBI GeneID: 3340
• NCBI Accession #: NP_001534.1
• NCBI GenBank Nucleotide #: NM_001543.4
• UniProt Accession #: P52848; Q96E57
• Molecular Weight: 62,065 Da
• NCBI Official Full Name: bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform 1
• NCBI Official Synonym Full Names: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
• NCBI Official Symbol: NDST1
• NCBI Official Synonym Symbols: HSST; NST1
• NCBI Protein Information: bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; NDST-1; HSNST 1; N-HSST 1; N-heparan sulfate sulfotransferase 1; glucosaminyl N-deacetylase/N-sulfotransferase 1; heparan sulfate-N-deacetylase/N-sulfotransferase; [Heparan sulfate]-glucosam
• UniProt Protein Name: Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
• UniProt Gene Name: NDST1
• UniProt Synonym Gene Names: HSST; HSST1; NDST-1; N-HSST 1; HSNST 1

NCBI Description

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:10758005, PubMed:12634318). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (PubMed:10758005, PubMed:12634318). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).