Rabbit MYCBP2 Polyclonal Antibody | anti-MYCBP2 antibody

MYCBP2 Antibody

Cat. Num. AAA9612417

• Gene Names: MYCBP2; PAM; Phr; PHR1; Myc-bp2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MYCBP2; Polyclonal Antibody; MYCBP2 Antibody; AU023734; AW107953; AW546647; C130061D10Rik; DKFZp686M08244; FLJ10106; FLJ13826; FLJ21597; FLJ21646; KIAA0916; MYC binding protein 2; PAM; Pam; highwire; rpm 1; Pam/highwire/rpm-1 protein; Phr1; Protein associated with Myc; R75243; anti-MYCBP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MYCBP2 Antibody detects endogenous levels of total MYCBP2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 4678

• Applicable Applications for anti-MYCBP2 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human MYCBP2, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using MYCBP2 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-MYCBP2 antibody

Function: Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues (PubMed:29643511). Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates (PubMed:29643511). Interacts with the E2 enzymes UBE2D1, UBE2D3, UBE2E1 and UBE2L3 (PubMed:18308511, PubMed:29643511). Plays a key role in neural development, probably by mediating ubiquitination of threonine residues on target proteins (Probable). Involved in different processes such as regulation of neurite outgrowth, synaptic growth, synaptogenesis and axon degeneration (By similarity). Required for the formation of major central nervous system axon tracts (By similarity). Required for proper axon growth by regulating axon navigation and axon branching: acts by regulating the subcellular location and stability of MAP3K12/DLK (By similarity). Required for proper localization of retinogeniculate projections but not for eye-specific segregation (By similarity). Regulates axon guidance in the olfactory system (By similarity). Involved in Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons: acts by promoting destabilization of NMNAT2, probably via ubiquitination of NMNAT2 (By similarity). Catalyzes ubiquitination of threonine and/or serine residues on NMNAT2, consequences of threonine and/or serine ubiquitination are however unknown (PubMed:29643511). Regulates the internalization of TRPV1 in peripheral sensory neurons (By similarity). Mediates ubiquitination and subsequent proteasomal degradation of TSC2/tuberin (PubMed:18308511, PubMed:27278822). Independently of the E3 ubiquitin-protein ligase activity, also acts as a guanosine exchange factor (GEF) for RAN in neurons of dorsal root ganglia (PubMed:26304119). May function as a facilitator or regulator of transcriptional activation by MYC (PubMed:9689053). Acts in concert with HUWE1 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1 (PubMed:20534529).

Post Translational Modifications: Autoubiquitinated.

Subcellular Location: Nucleus. Cell projection>Axon. Cytoplasm>Cytoskeleton. Note: Localizes to axon shafts and associates with microtubule cytoskeleton (By similarity). Translocates to the nucleus following interaction with sumoylated RANGAP1 (PubMed:26304119).

Tissue Specificity: Expressed in all tissues examined, expression is exceptionally abundant in brain and thymus. Colocalizes with TSC1 and TSC2 along the neurites and in the growth cones. Highly expressed in peripheral and central neurons. Colocalized with TSC1 in one of the filopodial extensions at the tip of a growth cone.

Subunit Structure: Interacts with MYC (PubMed:9689053). Interacts with TSC2 (tuberin) when TSC2 is in complex with TSC1 (hamartin) (PubMed:14559897). Interacts with FBXO45 (PubMed:19398581). Interacts with RAE1 (PubMed:22357847). Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Interacts with (sumoylated) RANGAP1; interaction with sumoylated RANGAP1 inhibits E3 ubiquitin-protein ligase activity and promotes MYCBP2 translocation to the nucleus (PubMed:26304119). Interacts with RAN (PubMed:26304119). Interacts with RAN (PubMed:26304119). Interacts with ATP13A2; the interaction inhibits the ubiquitination of TSC2 by MYCBP2 (PubMed:27278822).

Similarity: The PHR domains are compact beta-sandwich folds composed of 11 antiparallel strands and decorated with conserved apical loops. They are likely to play a structural role and mediate interactions with substrates or partners (By similarity).The tandem cysteine domain region confers threonine specificity and contains the two essential catalytic cysteine residues that relay ubiquitin. It binds four zinc ions in a C5HC7HC2 configuration.Belongs to the RING-Cys relay (RCR) family.

NCBI and Uniprot Product Information

• NCBI GI #: 291190787
• NCBI GeneID: 23077
• NCBI Accession #: NP_055872.4
• NCBI GenBank Nucleotide #: NP_055872.4
• UniProt Accession #: O75592
• Molecular Weight: Observed Molecular Weight: (Observed)510kD.; Predicted Molecular Weight: (Calculated)514kDa.
• NCBI Official Full Name: E3 ubiquitin-protein ligase MYCBP2
• NCBI Official Synonym Full Names: MYC binding protein 2
• NCBI Official Symbol: MYCBP2
• NCBI Official Synonym Symbols: PAM; Phr; PHR1; Myc-bp2
• NCBI Protein Information: E3 ubiquitin-protein ligase MYCBP2
• UniProt Protein Name: E3 ubiquitin-protein ligase MYCBP2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MYCBP2

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase and member of the PHR (Phr1/MYCBP2, highwire and RPM-1) family of proteins. The encoded protein plays a role in axon guidance and synapse formation in the developing nervous system. In mammalian cells, this protein regulates the cAMP and mTOR signaling pathways, and may additionally regulate autophagy. Reduced expression of this gene has been observed in acute lymphoblastic leukemia patients and a mutation in this gene has been identified in patients with a rare inherited vision defect. [provided by RefSeq, Mar 2017]

Uniprot Description

MYCBP2: Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. May function as a facilitator or regulator of transcriptional activation by MYC. May have a role during synaptogenesis. Belongs to the highwire family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 13q22.3

Cellular Component: membrane; nucleus

Molecular Function: metal ion binding; protein binding; transferase activity

Biological Process: protein ubiquitination; regulation of transcription, DNA-templated; transcription, DNA-dependent