Rabbit GGA3 Polyclonal Antibody | anti-GGA3 antibody

GGA3 Antibody

Cat. Num. AAA9612368

• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GGA3; Polyclonal Antibody; GGA3 Antibody; ADP ribosylation factor binding protein 3; ADP ribosylation factor binding protein GGA 3; ADP ribosylation factor binding protein GGA3; ADP-ribosylation factor-binding protein GGA3; ARF binding protein GGA 3; ARF binding protein GGA3; ARF-binding protein 3; gamma ear-containing; GGA 3; GGA3_HUMAN; Golgi associated gamma adaptin ear containing ARF binding protein 3; Golgi localized gamma ear containing ARF binding protein 3; Golgi-localized; KIAA0154; anti-GGA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GGA3 Antibody detects endogenous levels of total GGA3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 651

• Applicable Applications for anti-GGA3 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human GGA3, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from A431 cells, using GGA3 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from various samples, using GGA3 Antibody.Lane 1: HepG2 cells, treated with blocking peptide;Lane 2: HepG2 cells;Lane 3: COS;Lane 4: Hela cells.)

Related Product Information for anti-GGA3 antibody

Function: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed:11301005). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed:26811329). nvolved in BACE1 transport and sorting as well as regulation of BACE1 protein levels (PubMed:17553422, PubMed:15615712, PubMed:20484053). Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation (PubMed:15615712). Modulates BACE1 protein levels intedepently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination (PubMed:20484053). Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane (By similarity).

Post Translational Modifications: Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals.Ubiquitinated.Proteolytically cleaved during apoptosis by CASP3.

Subcellular Location: Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein. Early endosome membrane>Peripheral membrane protein. Recycling endosome membrane>Peripheral membrane protein.

Tissue Specificity: Ubiquitously expressed.

Subunit Structure: Monomer (Probable). Interacts with GGA1 and GGA2 (PubMed:14638859). Binds to clathrin and activated ARFs, such as ARF1, ARF5 and ARF6 (PubMed:11301005, PubMed:11950392). Binds RABEP1 and RABGEF1 (PubMed:12505986, PubMed:15143060). Interacts with the membrane proteins M6PR/CD-MPR and IGF2R/CI-MPR and the accessory proteins SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin (PubMed:10814529, PubMed:11387475, PubMed:12060753, PubMed:12213833, PubMed:14665628). Interacts with TSG101 and UBC (PubMed:14660606, PubMed:15039775). Interacts with ADRA2B (PubMed:26811329). Interacts with NTRK1; the interaction is independent of NTRK1 activation and ubiquitination (PubMed:26446845). Interacts (via VHS domain) with BACE1 (via DXXLL motif) (PubMed:14567678).

Similarity: The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.The unstructured hinge region contains clathrin-binding and an autoinhibitory (DXXLL) motifs.The GAE domain binds accessory proteins regulating GGAs function.Belongs to the GGA protein family.

NCBI and Uniprot Product Information

• NCBI GI #: 289577109
• NCBI GeneID: 23163
• NCBI Accession #: NP_001166174.1
• NCBI GenBank Nucleotide #: NP_001166174.1
• UniProt Accession #: Q9NZ52
• Molecular Weight: Observed Molecular Weight: (Observed)78kD.; Predicted Molecular Weight: (Calculated)78kDa.
• NCBI Official Full Name: ADP-ribosylation factor-binding protein GGA3 isoform 1
• NCBI Official Synonym Full Names: golgi associated, gamma adaptin ear containing, ARF binding protein 3
• NCBI Official Symbol: GGA3
• NCBI Protein Information: ADP-ribosylation factor-binding protein GGA3
• UniProt Protein Name: ADP-ribosylation factor-binding protein GGA3
• Protein Family: ADP-ribosylation factor-binding protein
• UniProt Gene Name: GGA3
• UniProt Synonym Gene Names: KIAA0154
• UniProt Entry Name: GGA3_HUMAN

NCBI Description

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

GGA3: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Monomer. Interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200. Interacts with GGA1 and GGA2. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1 and the accessory proteins SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Ubiquitously expressed. Belongs to the GGA protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: clathrin adaptor complex; endosome membrane; trans-Golgi network

Molecular Function: ADP-ribosylation factor binding; protein binding

Biological Process: vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process