Testing Data
Rubella Virus Antigens Antigen
Rubella Grade IV Antigen (HPV77)
Applications
ELISA
Synonyms
Rubella Virus Antigens; Rubella Grade IV Antigen (HPV77); Rubella Grade IV Antigen (Strain HPV77) >90% Viral Protein; Rubella Virus Antigens antigen
Host
Vero Cells
Specificity
Rubella Grade IV Antigen (Strain HPV77) >90% Viral Protein
Form/Format
Purified, Liquid; 10 mM Tris, 150 mM Sodium Chloride, 1.0 mM EDTA, pH 8.0 - 8.4
Gradient purification
Gradient purification
Applicable Applications for Rubella Virus Antigens antigen
ELISA
Application Notes
Each laboratory should determine an optimum working titer for use in its particular application.
Protein Concentration
BCA Assay: Acceptable Range: > 0.250 mg/mL
Testing Results: 0.356 mg/mL
OD 260/280nm Assay: Acceptable Range: 0.45-0.60 mg/mL
Testing Results: 0.53 mg/mL
Testing Results: 0.356 mg/mL
OD 260/280nm Assay: Acceptable Range: 0.45-0.60 mg/mL
Testing Results: 0.53 mg/mL
Buffer
Tris / EDTA Sucrose Buffer
pH 8.0 - 8.5
pH 8.0 - 8.5
Protein Concentration
0.5mg/mL
~90% Viral Protein
~90% Viral Protein
Activity
ELISA Assay: Reference Lot Number: M27034016
Testing Results: Midpoint Titer (test antigen): 208.457
Acceptable Range: -15% -+50% of reference Antigen % of Reference: 47.42%
Testing Results: Midpoint Titer (reference antigen): 141.401
Background ELISA Ratio: Acceptable <2; Test Result: 1.9
Sensitivity/Specificity Negative Control: Acceptable To Report; Test Result: Acceptable
Sensitivty/Specificity Medium Positive Control: Acceptable >3; Test Result: 15.1
Sensitivty/Specificity High Positive Control: Acceptable <8; Test Result: 24.6
Testing Results: Midpoint Titer (test antigen): 208.457
Acceptable Range: -15% -+50% of reference Antigen % of Reference: 47.42%
Testing Results: Midpoint Titer (reference antigen): 141.401
Background ELISA Ratio: Acceptable <2; Test Result: 1.9
Sensitivity/Specificity Negative Control: Acceptable To Report; Test Result: Acceptable
Sensitivty/Specificity Medium Positive Control: Acceptable >3; Test Result: 15.1
Sensitivty/Specificity High Positive Control: Acceptable <8; Test Result: 24.6
Inactivation
Product inactivated by UV irradiation and verified by Infectivity Testing (Plaque Assay)
Acceptable Range: 0 PFU/mL
Testing Results: 0 PFU/mL
Acceptable Range: 0 PFU/mL
Testing Results: 0 PFU/mL
Notes
Recommended sonication using Microprobe set to 10% Amplitude. Apply 3 - 1 second pulses with sample on ice.
Dry Ice Shipment
Extra charge may be applied to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at <-65°C. Avoid multiple freeeze thaw cycles.
Testing Data
Testing Data
Product Categories/Family for Rubella Virus Antigens antigen
Similar Products
Product Notes
The Rubella Virus Antigens (Catalog #AAA318651) is an Antigen produced from Vero Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rubella Virus Antigens can be used in a range of immunoassay formats including, but not limited to, ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Researchers should empirically determine the suitability of the Rubella Virus Antigens for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rubella Virus Antigens, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
