Rabbit SEC16A Polyclonal Antibody | anti-SEC16A antibody

SEC16A Antibody

Cat. Num. AAA9612251

• Gene Names: SEC16A; p250; SEC16L; KIAA0310
• Reactivity: Human; Predicted Reactivity: Bovine(88%), Sheep(88%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SEC16A; Polyclonal Antibody; SEC16A Antibody; FLJ26737; KIAA0310; OTTHUMP00000022589; OTTHUMP00000022592; OTTHUMP00000022593; p250; Protein SEC16 homolog A; Protein transport protein Sec16A; RP11 413M3.10; SEC 16; SEC 16A; SEC16; SEC16 homolog A (S. cerevisiae); SEC16 homolog A; SEC16L; anti-SEC16A antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Bovine(88%), Sheep(88%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SEC16A Antibody detects endogenous levels of total SEC16A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2334

• Applicable Applications for anti-SEC16A antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SEC16A, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using SEC16A Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-SEC16A antibody

Function: Acts as a molecular scaffold that plays a key role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed:17192411, PubMed:17005010, PubMed:17428803, PubMed:21768384, PubMed:22355596). Mediates the recruitment of MIA3/TANGO to ERES (PubMed:28442536). Regulates both conventional (ER/Golgi-dependent) and GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane (PubMed:28067262). Positively regulates the protein stability of E3 ubiquitin-protein ligases RNF152 and RNF183 and the ER localization of RNF183 (PubMed:29300766). Acts as a RAB10 effector in the regulation of insulin-induced SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the cell membrane in adipocytes (By similarity).

Subcellular Location: Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein. Cytoplasm>Perinuclear region. Cytoplasm>Cytosol. Microsome membrane. Note: SAR1A activity is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation (PubMed:17192411). Localizes to endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). MIA3 and LRRK2 are required for its proper localization to ERES (PubMed:25201882, PubMed:28442536, PubMed:19638414, PubMed:17428803, PubMed:22355596). Recruited to microsomal membrane in SAR1-dependent manner (PubMed:17428803).

Tissue Specificity: Ubiquitous. Expressed at higher levels in the pancreas.

Subunit Structure: SEC16A and SEC16B are each present in multiple copies in a heteromeric complex (PubMed:17192411, PubMed:22355596). Interacts with SEC23A (PubMed:17192411, PubMed:17428803). Interacts with RNF183 and RNF152 (PubMed:29300766). Interacts with LRRK2 (via ROC domain) (PubMed:25201882). Interacts with SEC13 (PubMed:25201882, PubMed:19638414, PubMed:17428803). Interacts with RAB10 (By similarity). Interacts with MIA3 (PubMed:28442536). Interacts with GORASP2 in response to ER stress (PubMed:28067262).

Similarity: Belongs to the SEC16 family.

NCBI and Uniprot Product Information

• NCBI GI #: 449083351
• NCBI GeneID: 9919
• NCBI Accession #: NP_001263347.1
• NCBI GenBank Nucleotide #: NP_001263347.1
• UniProt Accession #: O15027
• Molecular Weight: Observed Molecular Weight: (Observed)233kD.; Predicted Molecular Weight: (Calculated)252kDa.
• NCBI Official Full Name: protein transport protein Sec16A isoform 2
• NCBI Official Synonym Full Names: SEC16 homolog A, endoplasmic reticulum export factor
• NCBI Official Symbol: SEC16A
• NCBI Official Synonym Symbols: p250; SEC16L; KIAA0310
• NCBI Protein Information: protein transport protein Sec16A
• UniProt Protein Name: Protein transport protein Sec16A
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC16A
• UniProt Synonym Gene Names: KIAA0310; SEC16; SEC16L
• UniProt Entry Name: SC16A_HUMAN

NCBI Description

This gene encodes a protein that forms part of the Sec16 complex. This protein has a role in protein transport from the endoplasmic reticulum (ER) to the Golgi and mediates COPII vesicle formation at the transitional ER. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Feb 2013]

Uniprot Description

Function: Defines endoplasmic reticulum exit sites (ERES) and is required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for normal transitional endoplasmic reticulum (tER) organization. Ref.1 Ref.9

Subunit structure: SEC16A and SEC16B are each present in multiple copies in a heteromeric complex. Interacts with SEC23A. Ref.1

Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein

Potential. Note: SAR1A activity is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. Ref.1 Ref.9

Tissue specificity: Ubiquitous. Expressed at higher levels in the pancreas. Ref.1

Sequence similarities: Belongs to the SEC16 family.

Sequence caution: The sequence BAA20769.4 differs from that shown. Reason: Erroneous initiation. The sequence CAI13949.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI13951.1 differs from that shown. Reason: Erroneous gene model prediction.