Rabbit ORP1 Polyclonal Antibody | anti-OSBPL1A antibody

ORP1 Antibody

Cat. Num. AAA9612473

• Gene Names: OSBPL1A; ORP1; ORP-1; OSBPL1B
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(83%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(88%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ORP1; Polyclonal Antibody; ORP1 Antibody; FLJ10217; ORP 1; ORP-1; OSBL1_HUMAN; OSBP 8; OSBP L1; OSBP related protein 1; OSBP-related protein 1; OSBP8; OSBPL 1; OSBPL 1A; OSBPL 1B; OSBPL1; OSBPL1A; OSBPL1B; Oxysterol binding protein like 1A; Oxysterol binding protein like 1B; Oxysterol binding protein related protein 1; Oxysterol-binding protein-related protein 1; anti-OSBPL1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(83%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ORP1 Antibody detects endogenous levels of total ORP1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 568

• Applicable Applications for anti-OSBPL1A antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human ORP1, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain, using ORP1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-OSBPL1A antibody

Function: Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (By similarity). Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A (PubMed:16176980). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).

Subcellular Location: Late endosome. Note: Colocalizes with RAB7A, RAB9A and LAMP1 in late endosomes.

Subunit Structure: Interacts with VAPA (By similarity). Interacts with the GTP-bound form of RAB7A. Interacts with OAS1B (By similarity).

Similarity: Belongs to the OSBP family.

NCBI and Uniprot Product Information

• NCBI GI #: 335353788
• NCBI GeneID: 114876
• NCBI Accession #: NP_001229437.1
• NCBI GenBank Nucleotide #: NP_001229437.1
• UniProt Accession #: Q9BXW6
• Molecular Weight: Observed Molecular Weight: (Observed)108kD.; Predicted Molecular Weight: (Calculated)108kDa.
• NCBI Official Full Name: oxysterol-binding protein-related protein 1 isoform 3
• NCBI Official Synonym Full Names: oxysterol binding protein like 1A
• NCBI Official Symbol: OSBPL1A
• NCBI Official Synonym Symbols: ORP1; ORP-1; OSBPL1B
• NCBI Protein Information: oxysterol-binding protein-related protein 1
• UniProt Protein Name: Oxysterol-binding protein-related protein 1
• Protein Family: Oxysterol-binding protein-related protein
• UniProt Gene Name: OSBPL1A
• UniProt Synonym Gene Names: ORP1; OSBP8; OSBPL1; OSBPL1B; ORP-1; OSBP-related protein 1
• UniProt Entry Name: OSBL1_HUMAN

NCBI Description

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

OSBPL1A: Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3- phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Belongs to the OSBP family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: nucleoplasm; lysosomal membrane; cytoplasm; late endosome; intracellular; nucleus

Molecular Function: protein binding; cholesterol binding; phospholipid binding

Biological Process: vesicle-mediated transport; cholesterol metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; lipid transport