Rabbit COPS4 Polyclonal Antibody | anti-COPS4 antibody

COPS4 Antibody

Cat. Num. AAA9612476

• Gene Names: COPS4; CSN4; SGN4
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: COPS4; Polyclonal Antibody; COPS4 Antibody; CH4; COP9 (constitutive photomorphogenic) homolog subunit 4; COP9 complex homolog subunit 4; COP9 complex S4; COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis); COP9 constitutive photomorphogenic homolog subunit 4; COP9 signalosome complex subunit 4; COP9 signalosome subunit 4; CSN complex subunit 4; CSN4; CSN4 SCHPO; CSN4_HUMAN; DCH4; JAB1 containing signalosome subunit 4; JAB1-containing signalosome subunit 4; MGC94896; SGN4; Signalosome subunit 4; anti-COPS4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: COPS4 Antibody detects endogenous levels of total COPS4.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 352

• Applicable Applications for anti-COPS4 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human COPS4, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using COPS4 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-COPS4 antibody

Function: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. Also involved in the deneddylation of non-cullin subunits such as STON2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1, IRF8/ICSBP and SNAPIN, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle.

Subunit Structure: Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COPS6, COPS7 (COPS7A or COPS7B), COPS8 and COPS9 isoform 1 (PubMed:18850735, PubMed:26456823). In the complex, it probably interacts directly with COPS1, COPS2, COPS3, COPS5, COPS6, COPS7 (COPS7A or COPS7B) and COPS8 (PubMed:18850735). Interacts with TOR1A; the interaction is direct and associates TOR1A and SNAPIN with the CSN complex (PubMed:21102408). Interacts with STON2; controls STON2 neddylation levels (PubMed:21102408). Interacts with ERCC6 (PubMed:26030138).

Similarity: Belongs to the CSN4 family.

NCBI and Uniprot Product Information

• NCBI GI #: 384367993
• NCBI GeneID: 51138
• NCBI Accession #: NP_001244935.1
• NCBI GenBank Nucleotide #: NP_001244935.1
• UniProt Accession #: Q9BT78
• Molecular Weight: Observed Molecular Weight: (Observed)46kD.; Predicted Molecular Weight: (Calculated)46kDa.
• NCBI Official Full Name: COP9 signalosome complex subunit 4 isoform 2
• NCBI Official Synonym Full Names: COP9 signalosome subunit 4
• NCBI Official Symbol: COPS4
• NCBI Official Synonym Symbols: CSN4; SGN4
• NCBI Protein Information: COP9 signalosome complex subunit 4
• UniProt Protein Name: COP9 signalosome complex subunit 4
• UniProt Gene Name: COPS4
• UniProt Synonym Gene Names: CSN4; SGN4
• UniProt Entry Name: CSN4_HUMAN

NCBI Description

This gene encodes one of eight subunits composing COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]