Western Blot (WB)
(Western blot analysis of extracts from 293 cells (starved for 16 hours) untreated or EGF-treated (100 ng/ml), using MBS628680 Antibody (upper) or control M4696-05(lower).)
Rabbit mTOR Polyclonal Antibody
mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
mTOR; Polyclonal Antibody; phosphorylated (Ser2448) (mTAb1; Mammalian Target of Rapamycin; FRAP; RAFT); Anti -mTOR; anti-mTOR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of human mTOR only when phosphorylated at Ser2,448. Species Crossreactivity: Mouse and rat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-mTOR antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1,000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Dilution: Western Blot: 1:1,000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser2,448 of human mTOR.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from 293 cells (starved for 16 hours) untreated or EGF-treated (100 ng/ml), using MBS628680 Antibody (upper) or control M4696-05(lower).)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells (starved for 16 hours) untreated or EGF-treated (100 ng/ml), using MBS628680 Antibody (upper) or control M4696-05(lower).)
Related Product Information for anti-mTOR antibody
The mammalian target of rapamycin, mTOR, also know as FRAP or RAFT (1-3), is a serine/ threonine protein kinase. mTOR acts as a sensor for ATP, amino acids, balancing the availability of nutrients and cell growth (4,5). When sufficient nutrients are available, mediated by phosphatidic acid (6), mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor 4E-BP1. These events result in activation of the translation of specific mRNA subpopulations. mTOR is phosphorylated at serine 2,448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at serine 2,481 (6,7).
Product Categories/Family for anti-mTOR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
mTOR, partial
Protein Family
Similar Products
Product Notes
The mTOR (Catalog #AAA628680) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's mTOR can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1,000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:100. Researchers should empirically determine the suitability of the mTOR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mTOR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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