Rabbit 4E Binding Protein 1 Monoclonal Antibody | anti-EIF4EBP1 antibody

4E Binding Protein 1, phosphorylated (Thr37/46) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Cat. Num. AAA632360

• Gene Names: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316
• Reactivity: Drosophila, Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Supernatant; Ascites
• Synonyms: 4E Binding Protein 1; Monoclonal Antibody; phosphorylated (Thr37/46) (Protein Synthesis Initiation Factor 4E Binding Protein; eIF-4EBP1; PHAS-1; PHAS-I; Eukaryotic Translation Initiation Factor BP); Anti -4E Binding Protein 1; anti-EIF4EBP1 antibody

• Host: Rabbit
• Reactivity: Drosophila, Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9B142
• Specificity: Recognizes endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. May crossreact with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Species Crossreativity: human, mouse, rat, monkey, and D. melanogaster.
• Purity/Purification: Supernatant; Ascites
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-EIF4EBP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and Flow cytometry.; Dilution: Western Blot: 1:1000. For Western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunohistochemistry (Paraffin): 1:400 (Citrate/TBST); Immunofluorescence (IF-IC): 1:50; Flow Cytometry: 1:100
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 (KLH-coupled)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MBS632360)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lymphoma using MBS632360)

Western Blot (WB)

(Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody (upper) and MBS632360 (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis using MBS632360 on SignalSlide (TM) Phospho-Akt (Ser473) IHC Controls (paraffin-embedded LNCaP cells untreated (left) or LY294002-treated (right)).)

Immunohistochemistry (IHC)

(mmunohistochemical analysis of paraffin-embedded human colon carcinoma using MBS632360 in the presence of control peptide (left) or Phospho-4E-BP1 (Thr37/46) Blocking Peptide (right).)

Related Product Information for anti-EIF4EBP1 antibody

When bound to eIF4E, 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation.

PHAS-I, also known as eIF4E-BP1 and PHAS-II,-III (eIF4E-BP2, 3), are members of a family of proteins that regulate eukaryotic translation initiation which is mediated by the cap structure (m7GpppN, where N=any nucleotide) present at the 5í end of all cellular mRNAs, except organellar (1). The m7 cap is essential for the translation of most mRNA because it directs the translation machinery of the 5í end of the mRNA via its interaction with the cap binding protein, the translation initiation factor 4E(eIF4E) (2). eIF4E plays an principal role in determining global translation rates because its interaction with the cap facilitates the binding of the ribosome to the mRNA. Consistent with this role, eIF4E is required for cell cycle progression, exhibits anti-apoptotic activity and when overexpressed transforms cells (2). Interaction with PHAS proteins prevents incorporation of eIF4E into an active translation initiation complex and inhibits cap-dependent translation. However, this inhibitory effect is alleviated following phosphorylation of the PHAS proteins by a P13K-dependent pathway, involving signaling by the anti-apoptotic kinase Akt/PKB, as well as FRAP/mTOR (2). Rat PHAS-I has 117 amino acids with a apparent molecular weight of 22kD and is 93% identical to eIF-4E-BPI cloned from human placenta (3, 4). PHAS-I and ñII were found to have overlapping but different patterns of expression in tissues. PHAS-I is expressed in a wide variety of cell types with the highest being in two of the most insulin-responsive tissues, adipocytes and skeletal muscle (3). Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1. Increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II but that regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA (5). The PHAS-I initiation factor has 2-8 phosphorylation sites and is multiply phosphorylated by insulin-stimulated protein kinase(s) resulting in 8-10 phosphorylated isoforms in exponentially growing cells. Changes occur in the expression of these isoforms in response to stresses such as heat shock, and this may contribute to translation repression (6).

Product Categories/Family for anti-EIF4EBP1 antibody

Antibodies; Abs to Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 561630
• NCBI GeneID: 1978
• UniProt Accession #: Q13541; Q6IBN3; B2R502; D3DSW8
• Molecular Weight: 12,580 Da
• NCBI Official Full Name: 4E-binding protein 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E binding protein 1
• NCBI Official Symbol: EIF4EBP1
• NCBI Official Synonym Symbols: BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316
• NCBI Protein Information: eukaryotic translation initiation factor 4E-binding protein 1; OTTHUMP00000225572; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• Protein Family: Eukaryotic translation initiation factor 4E-binding protein
• UniProt Gene Name: EIF4EBP1
• UniProt Entry Name: 4EBP1_HUMAN

NCBI Description

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]

Uniprot Description

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; response to ethanol; TOR signaling pathway; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development