Rabbit anti-Human mTOR Polyclonal Antibody

mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)

Cat. Num. AAA630914

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: mTOR; Polyclonal Antibody; phosphorylated (Ser2448) (mTAb1; Mammalian Target of Rapamycin; FRAP; RAFT); Anti -mTOR; anti-mTOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes phosphorylated mTOR from human derived tissues and cells. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as liquid in PBS, 0.1% sodium azide. No stabilizing proteins added.

• Applicable Applications for anti-mTOR antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:15,000-1:65,000; Western Blot: 1:500-1:2000; Immunohistochemistry: 5ug/ml
• Immunogen: Synthetic peptide correspoding to aa2440-2457 of human mTOR.
• Preparation and Storage: Storage and Stability:; May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(MBS630914 was used (5ug/ml) to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. Tissue was FFPE. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)

Related Product Information for anti-mTOR antibody

Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR has a long list of synonyms including FK506 binding protein12-rapamycin associated protein 1, FK506 binding protein12-rapamycin associated protein 2, FRAP1, FRAP2, RAFT1, RAPT1 and/or FKBP12-rapamycin associated protein (FRAP). mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12- rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.

Product Categories/Family for anti-mTOR antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 433687128
• NCBI Official Full Name: mTOR, partial
• Protein Family: Serine/threonine-protein kinase