Rabbit MEK5 Polyclonal Antibody | anti-MEK5 antibody

MEK5(phospho-Ser311/ Thr315) Antibody

Cat. Num. AAA9411307

• Gene Names: Map2k5; Mek5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: MEK5; Polyclonal Antibody; MEK5(phospho-Ser311/ Thr315) Antibody; Map2k5; anti-MEK5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of MEK5 only when phosphorylated at serine 311 or threonine 315.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 448

• Applicable Applications for anti-MEK5 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S(p)I-A-K-T(p)-Y-V) derived from Rat MEK5.
• Modification: Phospho-Ser311/ Thr315
• Target Name: MEK5
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from Rat and Mouse brain tissue using MEK5(phospho-Ser311/ Thr315) Antibody.)

Related Product Information for anti-MEK5 antibody

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.

Product Categories/Family for anti-MEK5 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 77157802
• NCBI GeneID: 29568
• NCBI Accession #: NP_001029159.1
• NCBI GenBank Nucleotide #: NM_001033987.1
• UniProt Accession #: Q62862; Q62863; Q62864
• Molecular Weight: 40,091 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform a
• NCBI Official Synonym Full Names: mitogen activated protein kinase kinase 5
• NCBI Official Symbol: Map2k5
• NCBI Official Synonym Symbols: Mek5
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• UniProt Gene Name: Map2k5
• UniProt Synonym Gene Names: Mek5; Mkk5; Prkmk5; MAP kinase kinase 5; MAPKK 5; MEK 5
• UniProt Entry Name: MP2K5_RAT

NCBI Description

kinase involved in signal transduction in the cell [RGD, Feb 2006]

Uniprot Description

MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.

Protein type: Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); Kinase, protein; EC 2.7.12.2; STE group; STE7 family

Cellular Component: membrane; spindle; intracellular; nucleus; cytosol

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; protein amino acid phosphorylation; positive regulation of protein metabolic process; positive regulation of cell growth; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation