Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS645821. This demonstrates that MBS645821 detected the TCF4 protein (arrow).)
Rabbit anti-Human TCF4 Polyclonal Antibody | anti-TCF4 antibody
TCF4, NT (TCF4, BHLHB19, ITF2, SEF2, Transcription factor 4, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS645821. This demonstrates that MBS645821 detected the TCF4 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS645821. This demonstrates that MBS645821 detected the TCF4 protein (arrow).)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of K562 cells (right histogram) using MBS645821 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of K562 cells (right histogram) using MBS645821 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Aug 2011]
Uniprot Description
Function: Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription
By similarity. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
Subunit structure: Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2
By similarity.
Subcellular location: Nucleus Ref.13.
Tissue specificity: Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
Domain: the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.11
Involvement in disease: Pitt-Hopkins syndrome (PTHS) [MIM:610954]: A syndrome characterized by mental retardation, wide mouth and distinctive facial features, and intermittent hyperventilation followed by apnea. Features include intellectual disability with severe speech impairment, normal growth parameters at birth, postnatal microcephaly, breathing anomalies, severe motor developmental delay, motor incoordination, ocular anomalies, constipation, seizures, typical behavior and subtle brain abnormalities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19
Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.
Sequence caution: The sequence AAA60310.1 differs from that shown. Reason: Incomplete and probable erroneous sequence.The sequence AAA60312.1 differs from that shown. Reason: Incomplete and probable erroneous sequence.
Research Articles on TCF4
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Product Notes
The TCF4 tcf4 (Catalog #AAA645821) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF4, NT (TCF4, BHLHB19, ITF2, SEF2, Transcription factor 4, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCF4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the TCF4 tcf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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