Western Blot (WB)
(Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.)
Rabbit mDia2 Polyclonal Antibody | anti-mDia2 antibody
mDia2 (C-terminal region) Antibody
Gene Names
Diap3; Dia2; Drf3; mDia2; Diaph3; mKIAA4117; p134MDia2; 4930417P13Rik
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
mDia2; Polyclonal Antibody; mDia2 (C-terminal region) Antibody; mDia2 (C-terminal region); Dia2; anti-mDia2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was affinity purified using mDia2 (C-terminal region) peptide (without carrier). The antibody detects a 135kDa* protein corresponding to the apparent molecular mass of mDia2 on SDS-PAGE immunoblots of various cells and tissues, including human A431 and HeLa cells, mouse brain tissue and C2C12 cells, rat PC12 cells, and rabbit spleen fibroblasts.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-mDia2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:2000
WB: 1:1000
WB: 1:1000
Immunogen
mDia2 synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of mouse mDia2 (Diap3). This peptide sequence is highly conserved in rat and human mDia2, and has low homology to other formins.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.)
Western Blot (WB)
(Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.)
Related Product Information for anti-mDia2 antibody
Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia2 protein has been implicated in cell migration and cytokinesis. This Dia protein can nucleate actin polymerization, as well as bind and stabilize microtubules. mDia2 may also have functions in the nucleus, since it is continually shuttled between the cytoplasm and nucleus.
References
Miki, T. et al. (2009) J Biol Chem. 284:5753.
Bartolini, F. et al. (2008) J Cell Biol. 181:523.
Alberts, A.S. (2001) J Biol Chem. 276:2824.
Bartolini, F. et al. (2008) J Cell Biol. 181:523.
Alberts, A.S. (2001) J Biol Chem. 276:2824.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
133,686 Da
NCBI Official Full Name
protein diaphanous homolog 3
NCBI Official Synonym Full Names
diaphanous homolog 3 (Drosophila)
NCBI Official Symbol
Diap3
NCBI Official Synonym Symbols
Dia2; Drf3; mDia2; Diaph3; mKIAA4117; p134MDia2; 4930417P13Rik
NCBI Protein Information
protein diaphanous homolog 3; diaphanous-related formin; diaphanous-related formin-3
UniProt Protein Name
Protein diaphanous homolog 3
UniProt Gene Name
Diaph3
UniProt Synonym Gene Names
Diap3; DRF3; mDIA2
UniProt Entry Name
DIAP3_MOUSE
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Product Notes
The mDia2 diaph3 (Catalog #AAA474302) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mDia2 (C-terminal region) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's mDia2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:1000. Researchers should empirically determine the suitability of the mDia2 diaph3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mDia2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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