Western Blot (WB)
(Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4).)
Mouse anti-Human FHOD1 Monoclonal Antibody | anti-FHOD1 antibody
FHOD1 Antibody
Gene Names
FHOD1; FHOS
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Synonyms
FHOD1; Monoclonal Antibody; FHOD1 Antibody; anti-FHOD1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M352
Specificity
The antibody detects a 140kDa* protein corresponding to the apparent molecular mass of FHOD1 on SDS-PAGE immunoblots of human K562 and Jurkat, and has weak reactivity to FHOD1 in mouse C2C12.
Form/Format
Mouse monoclonal antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-FHOD1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:1000
IHC: 1:50
IHC: 1:50
IHC: 1:50
WB: 1:250
IHC: 1:50
IHC: 1:50
IHC: 1:50
WB: 1:250
Immunogen
FHOD1 recombinant protein that included the full length human FHOD1 sequence. This sequence has 50% homology to human FHOD3, and low homology to other formin family members.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4).)
Western Blot (WB)
(Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4).)
Related Product Information for anti-FHOD1 antibody
Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Several mammalian formins, including mDia1, FRL, and formin homology domain protein 1 (FHOD1) are inhibited through an intramolecular interaction between the C-terminal Dia autoregulatory domain (DAD) and its recognition region at the N-terminus. In FHOD1, this autoinhibitory interaction is disrupted through phosphorylation of Ser-1131, Ser-1137, and Thr-1141 by ROCK. Subsequent FHOD1 activation leads to stress fiber formation. In endothelial cells, thrombin activates this ROCK pathway, leading to FHOD1-mediated stress fiber formation.
References
Takeya, R. et al. (2008) EMBOJ 27:618.
Takeya, R. & Sumimoto, H. (2003) J Cell Sci. 116:4567.
Westendorf, J.J. (2001) J Biol Chem. 276:46453.
Takeya, R. & Sumimoto, H. (2003) J Cell Sci. 116:4567.
Westendorf, J.J. (2001) J Biol Chem. 276:46453.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126,551 Da
NCBI Official Full Name
FH1/FH2 domain-containing protein 1
NCBI Official Synonym Full Names
formin homology 2 domain containing 1
NCBI Official Symbol
FHOD1
NCBI Official Synonym Symbols
FHOS
NCBI Protein Information
FH1/FH2 domain-containing protein 1; formin homolog overexpressed in spleen 1
UniProt Protein Name
FH1/FH2 domain-containing protein 1
Protein Family
UniProt Gene Name
FHOD1
UniProt Synonym Gene Names
FHOS; FHOS1; FHOS
UniProt Entry Name
FHOD1_HUMAN
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Product Notes
The FHOD1 fhod1 (Catalog #AAA474365) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FHOD1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FHOD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). ELISA: 1:1000 IHC: 1:50 IHC: 1:50 IHC: 1:50 WB: 1:250. Researchers should empirically determine the suitability of the FHOD1 fhod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FHOD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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