Western Blot (WB)
(Western blot of Jurkat stimulated with calyculin A (100nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138; PX4825) (lanes 2 & 5) or phospho-Profilin (Ser-138; PX4795) (lanes 3 & 6) blocking peptides.)
Rabbit Profilin Polyclonal Antibody | anti-PFN1 antibody
Profilin (Ser-138), phospho-specific Antibody
Gene Names
PFN1; ALS18
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
Profilin; Polyclonal Antibody; Profilin (Ser-138); phospho-specific Antibody; phospho-specific; Profilin Ser138; anti-PFN1 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to unphosphorylated Profilin (Ser-138) then affinity purified using Profilin (Ser-138) peptide (without carrier). The antibody detects a 15kDa band corresponding to the apparent molecular mass of Profilin-1 in human Jurkat and A431 cells, as well as rat PC12 cells. This reactivity is decreased by lambda phosphatase treatment, and is blocked by phospho-profilin (Ser-138) blocking peptide.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-PFN1 antibody
Western Blot (WB), ELISA (EIA),
Application Notes
ELISA: 1:2000
WB: 1:1000
WB: 1:1000
Immunogen
Phospho-Profilin (Ser-138) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Ser-138 in human Profilin-1. This sequence is well conserved in rat and mouse Profilin-1. The serine site has some homology to the conserved site (Ser-138) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of Jurkat stimulated with calyculin A (100nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138; PX4825) (lanes 2 & 5) or phospho-Profilin (Ser-138; PX4795) (lanes 3 & 6) blocking peptides.)
Western Blot (WB)
(Western blot of Jurkat stimulated with calyculin A (100nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138; PX4825) (lanes 2 & 5) or phospho-Profilin (Ser-138; PX4795) (lanes 3 & 6) blocking peptides.)
Western Blot (WB)
(Western blot of human recombinant Profilin-1 phosphorylated in vitro with PKCalpha kinase then untreated (-) or treated with lambda phosphatase (+). The blots were probed with anti-Profilin (a.a. 126-137) (left panel) or anti-Profilin (Ser-138) phospho-specific (right panel) antibodies at 1:1000.)
Western Blot (WB)
(Western blot of human recombinant Profilin-1 phosphorylated in vitro with PKCalpha kinase then untreated (-) or treated with lambda phosphatase (+). The blots were probed with anti-Profilin (a.a. 126-137) (left panel) or anti-Profilin (Ser-138) phospho-specific (right panel) antibodies at 1:1000.)
Related Product Information for anti-PFN1 antibody
Profilins are small actin-binding proteins that have functions in cell motility, cytokinesis, gene transcription, endocytosis and neuronal plasticity. Four profilin isoforms have been identified in mammals. Profilin-1 (PFN1) and profilin-2a (PFN2a) isoforms are highly conserved in structure, but PFN1 is ubiquitously expressed while PFN2a is preferentially enriched in brain. In addition, there are two testis-specific profilins, PFN3 and PFN4, that significantly differ in primary sequence and function compared to PFN1 and PFN2a. Profilin is phosphorylated at both tyrosine and serine residues in vivo. Tyr-129 is phosphorylated in response to VEGF-A stimulation, and this promotes profilin actin binding and polymerization. Tyr-129 phosphorylation may be important for angiogenesis induced by injuries. Ser-138 is phosphorylated by ROCK and dephosphorylated by PP1. This serine phosphorylation inhibits G-actin binding, as well as decreases profilin's aggregation suppressor activity by inhibiting binding to huntingtin. Thus, Tyr-129 phosphorylation may activate while Ser-138 phosphorylation may inhibit profilin activity.
References
Shao, J. & Diamond, M.I. (2012) PLoS ONE 7(3): e32802.
Fan, Y. et al. (2012) Nat Cell Biol. 14(10):1046.
Shao, J. et al. (2008) Mol Cell Biol. 28(17):5196.
Fan, Y. et al. (2012) Nat Cell Biol. 14(10):1046.
Shao, J. et al. (2008) Mol Cell Biol. 28(17):5196.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
140
NCBI Official Full Name
profilin-1
NCBI Official Synonym Full Names
profilin 1
NCBI Official Symbol
PFN1
NCBI Official Synonym Symbols
ALS18
NCBI Protein Information
profilin-1; profilin I; epididymis tissue protein Li 184a
UniProt Protein Name
Profilin-1
Protein Family
UniProt Gene Name
PFN1
UniProt Entry Name
PROF1_HUMAN
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Product Notes
The PFN1 pfn1 (Catalog #AAA474541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Profilin (Ser-138), phospho-specific Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Profilin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA),. ELISA: 1:2000 WB: 1:1000. Researchers should empirically determine the suitability of the PFN1 pfn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Profilin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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