Western Blot (WB)
(Western blot analysis of COS-7 cells expressing DYKDDDDK-tagged mDia2 (lanes 1-3) or mDia2 mutant (T1061A) (lane 4). The cells were untreated (lane 1) or treated with calyculin A (lanes 2 & 3). The blot was treated with alkaline phosphatase (lane 2), then probed with anti-mDia2 (Thr-1061) (upper blot) or anti-DYKDDDDK antibody (lower blot). (Image provided by Dr. Christopher Mack in the Dept. of Pathology at UNC Chapel Hill).)
Rabbit mDia2 Polyclonal Antibody | anti-mDia2 antibody
mDia2 (Thr-1061), phospho-specific Antibody
Gene Names
Diap3; Dia2; Drf3; mDia2; Diaph3; mKIAA4117; p134MDia2; 4930417P13Rik
Reactivity
Human, Rat, Mouse, Chicken, Fish
Applications
Western Blot, ELISA
Synonyms
mDia2; Polyclonal Antibody; mDia2 (Thr-1061); phospho-specific Antibody; phospho-specific; Dia2 Thr1061; anti-mDia2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken, Fish
Clonality
Polyclonal
Specificity
This antibody was affinity purified using mDia2 (Thr-1061) peptide (without carrier). The antibody detects a 135kDa* protein corresponding to mDia2 in immunoblots of human A7r5 and mouse C2C12 cells treated with calyculin A. The antibody detects transfected mDia2 after treatment with calyculin A, but this reactivity is removed after phosphatase treatment. In addition, the antibody does not detect mDia2 (T1061A) mutant.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-mDia2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:2000
WB: 1:1000
WB: 1:1000
Immunogen
Phospho-mDia2 (Thr-1061) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-1061 in mouse mDia2 (Diap3). This peptide sequence is identical in rat and has 2 amino acid differences to human mDia2. The sequence has low homology to other formins.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of COS-7 cells expressing DYKDDDDK-tagged mDia2 (lanes 1-3) or mDia2 mutant (T1061A) (lane 4). The cells were untreated (lane 1) or treated with calyculin A (lanes 2 & 3). The blot was treated with alkaline phosphatase (lane 2), then probed with anti-mDia2 (Thr-1061) (upper blot) or anti-DYKDDDDK antibody (lower blot). (Image provided by Dr. Christopher Mack in the Dept. of Pathology at UNC Chapel Hill).)
Western Blot (WB)
(Western blot analysis of COS-7 cells expressing DYKDDDDK-tagged mDia2 (lanes 1-3) or mDia2 mutant (T1061A) (lane 4). The cells were untreated (lane 1) or treated with calyculin A (lanes 2 & 3). The blot was treated with alkaline phosphatase (lane 2), then probed with anti-mDia2 (Thr-1061) (upper blot) or anti-DYKDDDDK antibody (lower blot). (Image provided by Dr. Christopher Mack in the Dept. of Pathology at UNC Chapel Hill).)
Related Product Information for anti-mDia2 antibody
Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia2 protein has been implicated in cell migration and cytokinesis. This Dia protein can nucleate actin polymerization and stabilize microtubules. mDia2 may also function in the nucleus as the protein continually shuttles between the cytoplasm and nucleus. Regulation of mDia2 activity may occur through ROCK phosphorylation of Thr-1061 and Ser-1070. This phosphorylation disrupts inhibitory interactions between the DAD and DID domains leading to enhanced mDia2 activation of RhoA.
References
Staus, D.P. et al. (2011) Biochem J. 439(1):57-65.
Miki, T. et al. (2009) J Biol Chem. 284:5753.
Bartolini, F. et al. (2008) J Cell Biol. 181:523.
Alberts, A.S. (2001) J Biol Chem. 276:2824.
Miki, T. et al. (2009) J Biol Chem. 284:5753.
Bartolini, F. et al. (2008) J Cell Biol. 181:523.
Alberts, A.S. (2001) J Biol Chem. 276:2824.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
133,686 Da
NCBI Official Full Name
protein diaphanous homolog 3
NCBI Official Synonym Full Names
diaphanous homolog 3 (Drosophila)
NCBI Official Symbol
Diap3
NCBI Official Synonym Symbols
Dia2; Drf3; mDia2; Diaph3; mKIAA4117; p134MDia2; 4930417P13Rik
NCBI Protein Information
protein diaphanous homolog 3; diaphanous-related formin; diaphanous-related formin-3
UniProt Protein Name
Protein diaphanous homolog 3
UniProt Gene Name
Diaph3
UniProt Synonym Gene Names
Diap3; DRF3; mDIA2
UniProt Entry Name
DIAP3_MOUSE
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Product Notes
The mDia2 diaph3 (Catalog #AAA474305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mDia2 (Thr-1061), phospho-specific Antibody reacts with Human, Rat, Mouse, Chicken, Fish and may cross-react with other species as described in the data sheet. AAA Biotech's mDia2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:1000. Researchers should empirically determine the suitability of the mDia2 diaph3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mDia2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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