IgG1 Biotinylated Recombinant Protein | IgG1 recombinant protein

Recombinant Human IgG1 Fc Biotinylated

Cat. Num. AAA2569210

• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: IgG1 Biotinylated; Recombinant Human IgG1 Fc Biotinylated; g gamma-1 chain C region; IGHG1; IgG1 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Asp104-Lys330(Asp239Glu,Leu241Glu)

• Species: Human
• Tag: C-Avi
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Biotinylated Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330(Asp239Glu,Leu241Glu) is expressed with a Avi tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for IgG1 recombinant protein

As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50kDa) and two light chains (25kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcgammaR) on phagocytic cells with high affinity.

Product Categories/Family for IgG1 recombinant protein

immunology

NCBI and Uniprot Product Information

• NCBI GI #: 121039
• NCBI GeneID: 3500
• UniProt Accession #: P01857
• Molecular Weight: 150,000
• NCBI Official Full Name: Ig gamma-1 chain C region
• NCBI Official Synonym Full Names: immunoglobulin heavy constant gamma 1 (G1m marker)
• NCBI Official Symbol: IGHG1
• UniProt Protein Name: Ig gamma-1 chain C region
• UniProt Gene Name: IGHG1
• UniProt Entry Name: IGHG1_HUMAN

Uniprot Description

IGHG1: Defects in IGHG1 are a cause of multiple myeloma (MM). MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. A chromosomal aberration involving IGHG1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.

Protein type: Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; antigen binding

Biological Process: innate immune response; complement activation, classical pathway; complement activation

Disease: Myeloma, Multiple