Western Blot (WB)
(Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).)
Rabbit mDia3 Polyclonal Antibody | anti-mDia3 antibody
mDia3 (C-terminus) Antibody
WB: 1:1000
Western Blot (WB)
(Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).)
Western Blot (WB)
(Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).)
Lammers, M. et al. (2008) J Biol Chem. 283(50):35236.
Yasuda, S. et al. (2004) Nature. 428(6984):767.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Diaphanous-2: Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton. Isoform 3 interacts with RHOD in the GTP-bound form. Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes. Belongs to the formin homology family. Diaphanous subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: Xq21.33
Cellular Component: cytosol; early endosome; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; nucleolus
Molecular Function: actin binding; receptor binding; Rho GTPase binding
Biological Process: actin filament polymerization; cytokinesis; female gamete generation; multicellular organismal development; oogenesis; small GTPase mediated signal transduction
Disease: Premature Ovarian Failure 2a
Research Articles on mDia3
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Product Notes
The mDia3 diaph2 (Catalog #AAA474306) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mDia3 (C-terminus) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's mDia3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:1000. Researchers should empirically determine the suitability of the mDia3 diaph2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mDia3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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