Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta MARCKS representative staining pattern/morphology in rat placenta. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.)
Rabbit MARCKS Polyclonal Antibody | anti-LOC396473 antibody
MARCKS, phosphorylated (Ser152/156) (myristoylated alanine-rich protein kinase C substrate, 80K-L, 80K-L Protein, FLJ14368, FLJ90045, MACS, Myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, Protein kinase C substrate, 80kD Protein, Light Chain
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MARCKS; Polyclonal Antibody; phosphorylated (Ser152/156) (myristoylated alanine-rich protein kinase C substrate; 80K-L; 80K-L Protein; FLJ14368; FLJ90045; MACS; Myristoylated alanine-rich C-kinase substrate; PKCSL; PRKCSL; Protein kinase C substrate; 80kD Protein; Light Chain; Anti -MARCKS; anti-LOC396473 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat MARCKS when phosphorylated at Ser152/156.. Species Crossreactivity: mouse, human.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM sodium chloride, 100ug/mL BSA, 50% glycerol.
Applicable Applications for anti-LOC396473 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500
Immunohistochemistry (paraffin): 1:100
Dilution: Western Blot: 1:500
Immunohistochemistry (paraffin): 1:100
Immunogen
Synthetic phosphopeptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.
Positive Control
A431 Cell Lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta MARCKS representative staining pattern/morphology in rat placenta. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.)
Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta MARCKS representative staining pattern/morphology in rat placenta. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.)
Western Blot (WB)
(Western Blot Analysis: EGF treated A431 lysates were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-MARCKS PHOSPHO SER152/156 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MARCKS (~80 kD).)
Western Blot (WB)
(Western Blot Analysis: EGF treated A431 lysates were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-MARCKS PHOSPHO SER152/156 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MARCKS (~80 kD).)
Western Blot (WB)
(Western Blot Analysis: Representative image from a previous lot. pervanadate treated HEK293 lysate (Lane 1), pervanadate treated C2C12 lysate (Lane 2), pervanadate treated Huvec lysate (Lane 3), EGF treated A431 lysate (Lane 4), pervanadate treated L6 lysate (Lane 5) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-MARCKS PHOSPHO SER152/156 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MARCKS (~80kD).)
Western Blot (WB)
(Western Blot Analysis: Representative image from a previous lot. pervanadate treated HEK293 lysate (Lane 1), pervanadate treated C2C12 lysate (Lane 2), pervanadate treated Huvec lysate (Lane 3), EGF treated A431 lysate (Lane 4), pervanadate treated L6 lysate (Lane 5) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-MARCKS PHOSPHO SER152/156 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MARCKS (~80kD).)
Related Product Information for anti-LOC396473 antibody
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin. Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose).
Product Categories/Family for anti-LOC396473 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,728 Da
NCBI Official Full Name
myristoylated alanine-rich C-kinase substrate
NCBI Official Symbol
LOC396473
NCBI Protein Information
myristoylated alanine-rich C-kinase substrate; MARCKS
UniProt Protein Name
Myristoylated alanine-rich C-kinase substrate
Protein Family
UniProt Gene Name
MARCKS
UniProt Entry Name
MARCS_CHICK
Uniprot Description
MARCKS: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein
Protein type: Motility/polarity/chemotaxis; Actin-binding
Cellular Component: cytoskeleton; membrane; cytoplasm
Molecular Function: calmodulin binding; actin binding
Research Articles on LOC396473
Similar Products
Product Notes
The LOC396473 marcks (Catalog #AAA620589) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCKS, phosphorylated (Ser152/156) (myristoylated alanine-rich protein kinase C substrate, 80K-L, 80K-L Protein, FLJ14368, FLJ90045, MACS, Myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, Protein kinase C substrate, 80kD Protein, Light Chain reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MARCKS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:500 Immunohistochemistry (paraffin): 1:100. Researchers should empirically determine the suitability of the LOC396473 marcks for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCKS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
