Testing Data (TD)
( Overlay histogram showing Hela cells stained with AP6720b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody MBS9211992, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human MAPE Polyclonal Antibody | anti-PRAME antibody
MAPE Antibody (C-term)
WB~~1:2000
IHC-P~~ 1:50~100
Testing Data (TD)
( Overlay histogram showing Hela cells stained with AP6720b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody MBS9211992, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Testing Data (TD)
( Overlay histogram showing Hela cells stained with AP6720b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody MBS9211992, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Testing Data (TD)
( All lanes : Anti-MAPE Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: human testis lysate Lane 5: human brain lysate Lane 6: human kidney lysate Lysates/proteins at 20 ug perlane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 58 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
Testing Data (TD)
( All lanes : Anti-MAPE Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: human testis lysate Lane 5: human brain lysate Lane 6: human kidney lysate Lysates/proteins at 20 ug perlane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 58 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
Testing Data (TD)
( MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated)
Testing Data (TD)
( MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated)
Qin,Y., Leuk. Res. 33 (3), 384-390 (2009)
Neumann,E., Cancer Res. 58 (18), 4090-4095 (1998)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Uniprot Description
PRAME: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Belongs to the PRAME family.
Protein type: Transcription, coactivator/corepressor; Cancer Testis Antigen (CTA); Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 22q11.22
Cellular Component: plasma membrane; nucleus
Molecular Function: protein binding; retinoic acid receptor binding
Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; transcription, DNA-dependent; apoptosis; regulation of growth; positive regulation of cell proliferation; cell differentiation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Research Articles on PRAME
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Product Notes
The PRAME prame (Catalog #AAA9211992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 476-502. The MAPE Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAPE can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry ( FC ), Western Blot ( WB ), Immunohistochemistry-P ( IHC-P ), ELISA ( EIA ). FC~~ 1:25 WB~~1:2000 IHC-P~~ 1:50~100. Researchers should empirically determine the suitability of the PRAME prame for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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