Rabbit Factor X Polyclonal Antibody | anti-FX antibody

Factor X Antibody

Cat. Num. AAA5312277

• Gene Names: F10; fX; Cf10
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Antigen-Affinity Chromatography
• Synonyms: Factor X; Polyclonal Antibody; Factor X Antibody; Affinity Purified Rabbit Polyclonal Factor X Antibody; Polyclonal Factor X antibody; Anti-Factor X antibody; coagulation factor X antibody; F10 antibody; Stuart-Prower factor antibody; FX antibody; FXA antibody; prothrombinase antibody; factor Xa antibody; anti-FX antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen-Affinity Chromatography
• Form/Format: Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1

• Applicable Applications for anti-FX antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:100-1:500; ICC: 1:100-1:500; IHC-P: 1:100-1:500; WB: 1:1000-1:10000
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of methanol-fixed HeLa, using Factor X antibody at 1:200 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded A549 xenograft using Factor X antibody at a dilution of 1:500)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 10% SDS PAGE gel and Factor X antibody at a dilution of 1:5000)

Related Product Information for anti-FX antibody

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa, or by factor VIIa. The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity.

Product Categories/Family for anti-FX antibody

Nutrition & Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4322473
• NCBI GeneID: 14058
• Molecular Weight: 55 kDa
• NCBI Official Full Name: factor X, partial
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: fX; Cf10
• NCBI Protein Information: coagulation factor X
• Protein Family: Factor X-activator

NCBI Description

This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]