Western Blot (WB)
(Western BlotPositive WB detected in: A375 whole cell lysate, Mouse brain tissueAll lanes: PRAME antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 KDaObserved band size: 58 KDa)
Rabbit anti-Human Melanoma antigen preferentially expressed in tumors Polyclonal Antibody | anti-PRAME antibody
Rabbit anti-human Melanoma antigen preferentially expressed in tumors polyclonal Antibody
Western Blot (WB)
(Western BlotPositive WB detected in: A375 whole cell lysate, Mouse brain tissueAll lanes: PRAME antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 KDaObserved band size: 58 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: A375 whole cell lysate, Mouse brain tissueAll lanes: PRAME antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 KDaObserved band size: 58 KDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7002432 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7002432 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7002432 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7002432 at dilution 1:100)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with MBS7002432 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with MBS7002432 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Uniprot Description
PRAME: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Belongs to the PRAME family.
Protein type: Transcription, coactivator/corepressor; Cancer Testis Antigen (CTA); Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 22q11.22
Cellular Component: nucleus; plasma membrane
Molecular Function: protein binding; retinoic acid receptor binding
Biological Process: apoptosis; cell differentiation; negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of retinoic acid receptor signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; regulation of growth; transcription, DNA-dependent
Research Articles on PRAME
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Product Notes
The PRAME prame (Catalog #AAA7002432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Melanoma antigen preferentially expressed in tumors polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Melanoma antigen preferentially expressed in tumors can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). Recommended dilution: IHC: 1:20-1:200. Researchers should empirically determine the suitability of the PRAME prame for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Melanoma antigen preferentially expressed in tumors, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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