Rabbit anti-Human Lysine, Acetylated Polyclonal Antibody

Lysine, Acetylated (Acetyl-Lysine)

Cat. Num. AAA647194

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Lysine; Acetylated; Polyclonal Antibody; Acetylated (Acetyl-Lysine); Anti -Lysine; anti-Lysine; Acetylated antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes proteins post-translationally modified by acetylation on the epsilon-amino groups of lysine residues. Recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF and chemically acetylated BSA. Has been shown to react with as little as 0.04ng of chemically acetylated BSA while not recognizing up to 25ug of nonacetylated BSA.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-Lysine, Acetylated antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipation, Chromatin Immunoprecipitation, Immunohistcohemistry, Immunofluorescence and ELISA.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:100; Chromatin IP: 1:50; Immunohistochemistry (paraffin): 1:1600; Immunofluorescence (IF-IC): 1:200; ELISA (peptide): 1:1000
• Immunogen: Synthetic acetylated lysine-containing peptide
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from NIH/3T3 cells, untreated or sodium butyrate-treated (5mM for 24 hours), showing an increase in histone acetylation, using MBS647194.)

Western Blot (WB)

(Western Blot analysis of extracts from COS cells, untreated or TSA-treated, grown in 10% FBS (lanes 1 and 2) or serum starved for 18 hours (lanes 3 and 4), using MBS647194 (upper) or p44/42 MAP Kinase Antibody (lower).)

Testing Data

(Specificity and sensitivity of MBS647194 assayed on acetylated BSA (4; 1; 0.2; 0.04 or 0.008ng in lanes 1-5) or nonacetylated BSA (25,000; 5000; 1000 or 200ng in lanes 6–9).)

Immunofluorescence (IF)

(Immunofluorescent image of 293-T cells, showing nuclear localization of acetylated histones (red), using MBS647194. (Provided by Dr. Paul Digard, University of Cambridge, UK.))

Immunohistochemistry (IHC)

(IHC staining of a paraffin-embedded human breast tumor section, showing nuclear and cytoplasmic localization of proteins with acetylated lysine residues, using MBS647194.)

Related Product Information for anti-Lysine, Acetylated antibody

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).

Product Categories/Family for anti-Lysine, Acetylated antibody

Antibodies; Abs to Amino Acids