Rabbit Lysine, Acetylated Monoclonal Antibody

Lysine, Acetylated (Acetyl-Lysine)

Cat. Num. AAA6013224

• Reactivity: Bovine, C elegans, Canine, Chicken, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus0
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Purified; Purified
• Synonyms: Lysine; Acetylated; Monoclonal Antibody; Acetylated (Acetyl-Lysine); Anti -Lysine; anti-Lysine; Acetylated antibody

• Host: Rabbit
• Reactivity: Bovine, C elegans, Canine, Chicken, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus0
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 11C1044
• Specificity: Recognizes acetylated lysine in a wide range of sequence contexts. Species Crossreactivity: human, mouse, rat, hamster, monkey, mink, chicken, D. melanogaster, xenopus, zebrafish, bovine, canine, porcine, S. cerevisiae, C. elegans, horse.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Lysine, Acetylated antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: ELISA-Peptide: 1:1000; Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:100; Chromatin IP: 1:50
• Immunogen: Synthetic peptide containing Acetylated-Lysine library.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from COS and HeLa cells, untreated or TSA-treated (1uM, 6 hours), using Acetylated-Lysine Rabbit mAb #MBS6013224.)

Western Blot (WB)

(Western blot analysis of extracts from various mouse tissues using Acetylated-Lysine Rabbit mAb #MBS6013224.)

Immunoprecipitation (IP)

(Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 HeLa cells and either 10ul of Acetylated-Lysine MBS6013224 or 2ul of Normal Rabbit IgG, using Enzymatic Chromatin IP Kit (Magnetic Beads). The enriched DNA was quantified by real-time PCR using Human GAPDH Exon 1 Primers, Human RPL30 Exon 3 Primers, Human MyoD1 Exon 1 Primers, and Human α)

Related Product Information for anti-Lysine, Acetylated antibody

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/ deacetylation of histones, transcription factors and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin

Product Categories/Family for anti-Lysine, Acetylated antibody

Antibodies; Abs to Amino Acids