Testing Data
Rabbit Rabex-5 Polyclonal Antibody | anti-RABGEF1 antibody
Rabex-5 Antibody
Appearance: Colorless liquid
Shelf Life: 12 months
Testing Data
Testing Data
Background: Rabex-5, also called RabGEF1 and RAP1, was identified as a guanine nucleotide exchange factor (GEF) for Rab5, a member of the Ras superfamily of small Rab GTPases (1). Rabex-5 generates the GTP-bound active form of Rab5 and forms a tight association with its effector protein Rabaptin-5. This complex localizes to endosomal membranes where it functions as a key regulator of vesicular trafficking during early endocytosis. Rabex-5 is also monoubiquitinated and has ubiquitin ligase activity that regulates its recruitment to early endosomes. The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis. Through its control of endosomal trafficking and endocytosis, Rabex-5 has been shown to negatively regulate NGF-mediated neurite outgrowth as well as FcepsilonRI-dependent mast cell activation.
NCBI and Uniprot Product Information
NCBI Description
RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010]
Uniprot Description
RABGEF1: Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase. Interacts with RGS14; the interaction is GTP-dependent. Heterodimer with RABEP1. The heterodimer binds RAB4A and RAB5A that have been activated by GTP-binding. Interacts with RAB21, and with 100-fold lower affinity also with RAB22. Binds TSC2, GGA1, GGA2, GGA3, AP1G1 and AP1G2. Interacts with ubiquitinated EGFR. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; GAPs, Rab
Chromosomal Location of Human Ortholog: 7q11.21
Cellular Component: recycling endosome; early endosome; vesicle
Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; Rab guanyl-nucleotide exchange factor activity; Rab GTPase binding
Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of mast cell degranulation; negative regulation of inflammatory response; protein ubiquitination; negative regulation of Ras protein signal transduction; endocytosis; negative regulation of receptor-mediated endocytosis; protein targeting to membrane; negative regulation of leukocyte migration
Research Articles on RABGEF1
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Product Notes
The RABGEF1 rabgef1 (Catalog #AAA841401) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabex-5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rabex-5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blot: 1:200. Researchers should empirically determine the suitability of the RABGEF1 rabgef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rabex-5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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