Western Blot (WB)
(Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).)
Rabbit Acetylated Lysine Polyclonal Antibody
Acetylated Lysine Antibody
Reactivity
Acetylated lysine residues. No reaction to non-acetylated proteins.
Applications
Western Blot, Immunoprecipitation, ELISA, Immunofluorescence
Synonyms
Acetylated Lysine; Polyclonal Antibody; Acetylated Lysine Antibody; lysine; acetyl lysine; anti-Acetylated Lysine antibody
Host
Rabbit
Reactivity
Acetylated lysine residues. No reaction to non-acetylated proteins.
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-Acetylated Lysine antibody
Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunofluorescence (IF)
Application Notes
1:250 (WB)
Certificate of Analysis
A 1/250 dilution of SPC-155 was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis.
Conjugate
Unconjugated
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).)
Western Blot (WB)
(Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).)
Western Blot (WB)
(Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:1000.)
Western Blot (WB)
(Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155). Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody (SPC-155) at 1:1000.)
Related Product Information for anti-Acetylated Lysine antibody
Background Info: Detects proteins containing acetylated lysine residues in SDS PAGE immunoblots.
Scientific Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nepsilon-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
Scientific Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nepsilon-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
Product Categories/Family for anti-Acetylated Lysine antibody
References
1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218. 6. Chan, H.M. et al. (2001). Nat. Cell Biol. 3: 667-. 674. 7. Martinez-Balbas, M.A. et al. (2000). EMBO J. 19: 662-671.
1. Ishiguro, K., Ando, T., Maeda, O., Watanabe, O. and Goto, H. (2011). Cutting Edge: Tubulin α Functions as an Adaptor in NFAT–Importin β Interaction. J Immunol. 186 (5), 2710-2713. doi: 10.4049/jimmunol.1003322
1. Ishiguro, K., Ando, T., Maeda, O., Watanabe, O. and Goto, H. (2011). Cutting Edge: Tubulin α Functions as an Adaptor in NFAT–Importin β Interaction. J Immunol. 186 (5), 2710-2713. doi: 10.4049/jimmunol.1003322
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Product Notes
The Acetylated Lysine (Catalog #AAA805679) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acetylated Lysine Antibody reacts with Acetylated lysine residues. No reaction to non-acetylated proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Acetylated Lysine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunofluorescence (IF). 1:250 (WB). Researchers should empirically determine the suitability of the Acetylated Lysine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acetylated Lysine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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