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Western Blot (WB) (Western Blot: Sample: Mouse Pancreas lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse MRP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Macrophage Inflammatory Protein Related Protein 1 (MRP1) Polyclonal Antibody | anti-MRP1 antibody

Polyclonal Antibody to Macrophage Inflammatory Protein Related Protein 1 (MRP1)

Gene Names
Ccl6; c10; MRP-1; Scya6
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Macrophage Inflammatory Protein Related Protein 1 (MRP1); Polyclonal Antibody; Polyclonal Antibody to Macrophage Inflammatory Protein Related Protein 1 (MRP1); anti-MRP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MRP1. It has been selected for its ability to recognize MRP1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GLIQEMEKE DRRYNPPIIH QGFQDTSSDC CFSYATQIPC KRFIYYFPTS GGCIKPGIIF ISRRGTQVCA DPSDRRVQRC
Applicable Applications for anti-MRP1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MRP1 (Gly22~Ala116) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051060
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Pancreas lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse MRP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Pancreas lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse MRP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Spleen tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Brain Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Brain Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,984 Da
NCBI Official Full Name
C-C motif chemokine 6
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 6
NCBI Official Symbol
Ccl6
NCBI Official Synonym Symbols
c10; MRP-1; Scya6
NCBI Protein Information
C-C motif chemokine 6
UniProt Protein Name
C-C motif chemokine 6
Protein Family
UniProt Gene Name
Ccl6
UniProt Synonym Gene Names
C10; Scya6

Uniprot Description

CCL6: Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11 C|11 50.85 cM

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: cellular response to interferon-gamma; cellular response to interleukin-1; cellular response to tumor necrosis factor; chemokine-mediated signaling pathway; chemotaxis; G-protein coupled receptor signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of ERK1 and ERK2 cascade; positive regulation of GTPase activity

Research Articles on MRP1

Similar Products

Product Notes

The MRP1 ccl6 (Catalog #AAA2013277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Macrophage Inflammatory Protein Related Protein 1 (MRP1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Macrophage Inflammatory Protein Related Protein 1 (MRP1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MRP1 ccl6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GLIQEMEKE DRRYNPPIIH QGFQDTSSDC CFSYATQIPC KRFIYYFPTS GGCIKPGIIF ISRRGTQVCA DPSDRRVQRC. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory Protein Related Protein 1 (MRP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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