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Flow Cytometry (FC/FACS)

Rabbit anti-Human KIAA0141/DELE1 Polyclonal Antibody | anti-DELE1 antibody

Anti-KIAA0141/DELE1 Antibody

Gene Names
DELE1; DELE; KIAA0141
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
KIAA0141/DELE1; Polyclonal Antibody; Anti-KIAA0141/DELE1 Antibody; DAP3-binding cell death enhancer 1; Death ligand signal enhancer; DELE1; DELE; KIAA0141; anti-DELE1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
515
Applicable Applications for anti-DELE1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Human)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x10^6 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Other applications have not been tested.
Other dilutions should be determined by end users.
Immunogen
E Coli-derived human KIAA0141/DELE1 recombinant protein (Position: E218-L430).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Background
DAP3-binding cell death enhancer 1 is a protein that in humans is encoded by the DELE1 gene. This gene is mapped to 5q13.3.
Preparation and Storage
Store at -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen store at -20°C for a longer time.
Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Immunofluorescence (IF)

Immunofluorescence (IF)

Western Blot (WB)

(Figure 1. Western blot analysis of DELE1 using anti-DELE1 antibody (A14579-1).)

Western Blot (WB) (Figure 1. Western blot analysis of DELE1 using anti-DELE1 antibody (A14579-1).)
Related Product Information for anti-DELE1 antibody
Description: Rabbit IgG polyclonal antibody for KIAA0141/DELE1 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human.

Background: DAP3-binding cell death enhancer 1 is a protein that in humans is encoded by the DELE1 gene. This gene is mapped to 5q31.3.
References
1. Harada, T., Iwai, A., Miyazaki, T. Identification of DELE, a novel DAP3-binding protein which is crucial for death receptor-mediated apoptosis induction. Apoptosis 15: 1247-1255, 2010.
2. Hartz, P. A. Personal Communication. Baltimore, Md. 4/15/2014.
3. Nagase, T., Seki, N., Tanaka, A., Ishikawa, K., Nomura, N. Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 2: 167-174, 1995.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
death ligand signal enhancer
NCBI Official Synonym Full Names
DAP3 binding cell death enhancer 1
NCBI Official Symbol
DELE1
NCBI Official Synonym Symbols
DELE; KIAA0141
NCBI Protein Information
death ligand signal enhancer
UniProt Protein Name
Death ligand signal enhancer
UniProt Gene Name
KIAA0141
UniProt Synonym Gene Names
DELE
UniProt Entry Name
DELE_HUMAN

Uniprot Description

KIAA0141: Essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation.

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: mitochondrion

Molecular Function: protein binding

Biological Process: induction of apoptosis via death domain receptors; regulation of caspase activity

Research Articles on DELE1

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Product Notes

The DELE1 kiaa0141 (Catalog #AAA1753174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-KIAA0141/DELE1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KIAA0141/DELE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Human) ICC: 2ug/ml (Human) IF: 2ug/ml (Human) FC/FACS: 1-3ug/1x10^6 cells (Human) Direct ELISA: 0.1-0.5ug/ml (Human) Tested Species: In-house tested species with positive results. Other applications have not been tested. Other dilutions should be determined by end users. Researchers should empirically determine the suitability of the DELE1 kiaa0141 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIAA0141/DELE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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