Rabbit anti-Human RICTOR Polyclonal Antibody | anti-RICTOR antibody

Anti-RICTOR Antibody

Cat. Num. AAA1753071

• Gene Names: RICTOR; PIA; AVO3; hAVO3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: RICTOR; Polyclonal Antibody; Anti-RICTOR Antibody; Rapamycin-insensitive companion of mTOR; AVO3 homolog; hAVO3; KIAA1999; anti-RICTOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1708

• Applicable Applications for anti-RICTOR antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human); IHC-P (Embedded Section): 0.5-1ug/ml (Human); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human RICTOR recombinant protein (Position: E23-H264).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Immunofluorescence (IF)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

(Figure 1. Western blot analysis of RICTOR using anti-RICTOR antibody.)

Related Product Information for anti-RICTOR antibody

Description: Rabbit IgG polyclonal antibody for RICTOR detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.

Background: Rapamycin-insensitive companion of mTOR is a protein that in human is encoded by the RICTOR gene. This gene is mapped to 5p13.1. RICTOR and MTOR are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.

References

1. Sarbassov, D. D., Ali, S. M., Kim, D.-H., Guertin, D. A., Latek, R. R., Erdjument-Bromage, H., Tempst, P., Sabatini, D. M. Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Curr. Biol. 14: 1296-1302, 2004.
2. Sarbassov, D. D., Guertin, D. A., Ali, S. M., Sabatini, D. M. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307: 1098-1101, 2005.
3. Shiota, C., Woo, J.-T., Lindner, J., Shelton, K. D., Magnuson, M. A. Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability. Dev. Cell 11: 583-589, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 59891448
• NCBI GeneID: 253260
• NCBI Accession #: NP_689969.2
• NCBI GenBank Nucleotide #: NP_689969.2
• UniProt Accession #: Q6R327
• NCBI Official Full Name: rapamycin-insensitive companion of mTOR isoform 1
• NCBI Official Synonym Full Names: RPTOR independent companion of MTOR complex 2
• NCBI Official Symbol: RICTOR
• NCBI Official Synonym Symbols: PIA; AVO3; hAVO3
• NCBI Protein Information: rapamycin-insensitive companion of mTOR
• UniProt Protein Name: Rapamycin-insensitive companion of mTOR
• Protein Family: Rapamycin-insensitive companion
• UniProt Gene Name: RICTOR
• UniProt Synonym Gene Names: hAVO3
• UniProt Entry Name: RICTR_HUMAN

NCBI Description

RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]

Uniprot Description

RICTOR: a regulatory binding partner of the kinase mTOR. Part of a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Rictor interacts with Cullin1-Rbx1 to form an E3 ubiquitin ligase complex, and promotes ubiquitination and degradation of SGK1. Shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.

Protein type: Cell cycle regulation; Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: TORC2 complex; cytosol

Molecular Function: protein binding; ribosome binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; actin cytoskeleton reorganization; innate immune response; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway