Rabbit anti-Human, Mouse UBR2 Polyclonal Antibody | anti-UBR2 antibody

Anti-UBR2 Antibody

Cat. Num. AAA1753125

• Gene Names: UBR2; C6orf133; bA49A4.1; dJ242G1.1; dJ392M17.3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: UBR2; Polyclonal Antibody; Anti-UBR2 Antibody; E3 ubiquitin-protein ligase UBR2; N-recognin-2; RING-type E3 ubiquitin transferase UBR2; Ubiquitin-protein ligase E3-alpha-2; Ubiquitin-protein ligase E3-alpha-II; C6orf133; KIAA0349; anti-UBR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1755

• Applicable Applications for anti-UBR2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.1-0.25ug/ml (Human, Mouse); IHC-P (Embedded Section): 0.5-1ug/ml (Human); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human UBR2 recombinant protein (Position: Q10-A664).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Immunofluorescence (IF)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

(Figure 1. Western blot analysis of UBR2 using anti-UBR2 antibody.)

Related Product Information for anti-UBR2 antibody

Description: Rabbit IgG polyclonal antibody for UBR2 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse.

Background: E3 ubiquitin-protein ligase UBR2 is an enzyme that in humans is encoded by the UBR2 gene. It is mapped to 6p21.1. This gene encodes an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation. Alternative splicing results in multiple transcript variants.

References

1. Kwon, Y. T., Xia, Z., An, J. Y., Tasaki, T., Davydov, I. V., Seo, J. W., Sheng, J., Xie, Y., Varshavsky, A. Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway. Molec. Cell. Biol. 23: 8255-8271, 2003.
2. Nagase, T., Ishikawa, K., Nakajima, D., Ohira, M., Seki, N., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 4: 141-150, 1997.
3. Yin, J., Kwon, Y. T., Varshavsky, A., Wang, W. RECQL4, mutated in the Rothmund-Thomson and RAPADILINO syndromes, interacts with ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway. Hum. Molec. Genet. 13: 2421-2430, 2004.

NCBI and Uniprot Product Information

• NCBI GI #: 73622073
• NCBI GeneID: 23304
• UniProt Accession #: Q8IWV8
• NCBI Official Full Name: E3 ubiquitin-protein ligase UBR2
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 2
• NCBI Official Symbol: UBR2
• NCBI Official Synonym Symbols: C6orf133; bA49A4.1; dJ242G1.1; dJ392M17.3
• NCBI Protein Information: E3 ubiquitin-protein ligase UBR2
• UniProt Protein Name: E3 ubiquitin-protein ligase UBR2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: UBR2
• UniProt Synonym Gene Names: C6orf133; KIAA0349
• UniProt Entry Name: UBR2_HUMAN

NCBI Description

This gene encodes an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

UBR2: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. Belongs to the UBR1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; plasma membrane; chromatin; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of TOR signaling pathway; male meiosis I; chromatin silencing; spermatogenesis; histone H2A ubiquitination