Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit | FGF21 elisa kit

Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit

Cat. Num. AAA454816

• Reactivity: Mouse
• Synonyms: Fibroblast Growth Factor 21 (FGF21); Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit; FGF21 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGF21. No significant cross-reactivity or interference between FGF21 and analogues was observed.
• Sequence Length: 210

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 39.54pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF21 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF21 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for FGF21 elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FGF21 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to FGF21 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FGF21 and unlabeled FGF21 (Standards or samples) with the pre-coated antibody specific to FGF21. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FGF21 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FGF21 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 9910218
• NCBI GeneID: 56636
• NCBI Accession #: NP_064397.1
• NCBI GenBank Nucleotide #: NM_020013.4
• UniProt Accession #: Q9JJN1
• Molecular Weight: 23,237 Da
• NCBI Official Full Name: fibroblast growth factor 21
• NCBI Official Synonym Full Names: fibroblast growth factor 21
• NCBI Official Symbol: Fgf21
• NCBI Protein Information: fibroblast growth factor 21
• UniProt Protein Name: Fibroblast growth factor 21
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf21
• UniProt Synonym Gene Names: FGF-21

Uniprot Description

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB.