Rabbit Slc25a1 Polyclonal Antibody | anti-SLC25A1 antibody

Anti-Slc25a1 Antibody

Cat. Num. AAA1753128

• Gene Names: SLC25A1; CTP; SEA; CMS23; D2L2AD; SLC20A3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Slc25a1; Polyclonal Antibody; Anti-Slc25a1 Antibody; Tricarboxylate transport protein; mitochondrial; Citrate transport protein; CTP; Solute carrier family 25 member 1; Tricarboxylate carrier protein; SLC25A1; SLC20A3; anti-SLC25A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 311

• Applicable Applications for anti-SLC25A1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human, Mouse, Rat); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human Slc25a1 recombinant protein (Position: I38-D311).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Immunofluorescence (IF)

Western Blot (WB)

(Figure 1. Western blot analysis of SLC25A1 using anti-SLC25A1 antibody.)

Related Product Information for anti-SLC25A1 antibody

Description: Rabbit IgG polyclonal antibody for Slc25a1 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.

Background: Tricarboxylate transport protein, mitochondrial, also known as tricarboxylate carrier protein and citrate transport protein (CTP), is a protein that in humans is encoded by the SLC25A1 gene. It is mapped to 22q11.21. This gene encodes a member of the mitochondrial carrier subfamily of solute carrier proteins. Members of this family include nuclear-encoded transporters that translocate small metabolites across the mitochondrial membrane. This protein regulates the movement of citrate across the inner membranes of the mitochondria. Mutations in this gene have been associated with combined D-2- and L-2-hydroxyglutaric aciduria. Pseudogenes of this gene have been identified on chromosomes 7, 11, 16, and 19. Alternative splicing results in multiple transcript variants.

References

1. Chaouch, A., Porcelli, V., Cox, D., Edvardson, S., Scarcia, P., De Grassi, A., Pierri, C. L., Cossins, J., Laval, S. H., Griffin, H., Muller, J. S., Evangelista, T., and 13 others. Mutations in the mitochondrial citrate carrier SLC25A1 are associated with impaired neuromuscular transmission. J. Neuromusc. Dis. 1: 75-90, 2014.
2. Edvardson, S., Porcelli, V., Jalas, C., Sioferman, D., Kelner, Y., Shaaq, A., Korman, S. H., Pierri, C. L., Scarcia, P., Fraenkel, N. D., Segel, R., Schechter, A., Frumkin, A., Pines, O., Saada, A., Palmieri,., Elpeleg, O. Agenesis of corpus callosum and optic nerve hypoplasia due to mutations in SLC25A1 encoding the mitochondrial citrate transporter. J. Med. Genet. 50: 240-245, 2013.
3. Goldmuntz, E., Wang, Z., Roe, B. A., Budarf, M. L. Cloning, genomic organization, and chromosomal localization of human citrate transport protein to the DiGeorge/velocardiofacial syndrome minimal critical region. Genomics 33: 271-276, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 21389315
• NCBI GeneID: 6576
• NCBI Accession #: NP_005975
• NCBI GenBank Nucleotide #: NP_005975.1
• UniProt Accession #: P53007
• NCBI Official Full Name: tricarboxylate transport protein, mitochondrial isoform a
• NCBI Official Synonym Full Names: solute carrier family 25 member 1
• NCBI Official Symbol: SLC25A1
• NCBI Official Synonym Symbols: CTP; SEA; CMS23; D2L2AD; SLC20A3
• NCBI Protein Information: tricarboxylate transport protein, mitochondrial
• UniProt Protein Name: Tricarboxylate transport protein, mitochondrial
• UniProt Gene Name: SLC25A1
• UniProt Synonym Gene Names: SLC20A3; CTP
• UniProt Entry Name: TXTP_HUMAN

NCBI Description

This gene encodes a member of the mitochondrial carrier subfamily of solute carrier proteins. Members of this family include nuclear-encoded transporters that translocate small metabolites across the mitochondrial membrane. This protein regulates the movement of citrate across the inner membranes of the mitochondria. Mutations in this gene have been associated with combined D-2- and L-2-hydroxyglutaric aciduria. Pseudogenes of this gene have been identified on chromosomes 7, 11, 16, and 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]