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Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysateAll lanes: ZRANB1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 81 kDaObserved band size: 81 kDa)

Rabbit anti-Human ZRANB1 Polyclonal Antibody | anti-ZRANB1 antibody

ZRANB1 Antibody

Gene Names
ZRANB1; TRABID
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
ZRANB1; Polyclonal Antibody; ZRANB1 Antibody; Ubiquitin thioesterase ZRANB1; EC 3.4.19.12; TRAF-binding domain-containing protein; hTrabid; Zinc finger Ran-binding domain-containing protein 1; TRABID; anti-ZRANB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
708
Applicable Applications for anti-ZRANB1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:5000
IHC: 1:200-1:500
Immunogen
Recombinant Human Ubiquitin thioesterase ZRANB1 protein (111-397AA)
Conjugate
Non-Conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Species
Homo sapiens (Human)
Research Area
Cell Biology
Target Names
ZRANB1
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysateAll lanes: ZRANB1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 81 kDaObserved band size: 81 kDa)

Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysateAll lanes: ZRANB1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 81 kDaObserved band size: 81 kDa)

Immunohistochemistry (IHC)

(IHC image of MBS7114618 diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7114618 diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS7114618 diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7114618 diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Related Product Information for anti-ZRANB1 antibody
Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling.
Product Categories/Family for anti-ZRANB1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiquitin thioesterase ZRANB1
NCBI Official Synonym Full Names
zinc finger RANBP2-type containing 1
NCBI Official Symbol
ZRANB1
NCBI Official Synonym Symbols
TRABID
NCBI Protein Information
ubiquitin thioesterase ZRANB1
UniProt Protein Name
Ubiquitin thioesterase ZRANB1
Protein Family
UniProt Gene Name
ZRANB1
UniProt Synonym Gene Names
TRABID; hTrabid

Uniprot Description

Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling.

Research Articles on ZRANB1

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Product Notes

The ZRANB1 zranb1 (Catalog #AAA7114618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZRANB1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZRANB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:5000 IHC: 1:200-1:500. Researchers should empirically determine the suitability of the ZRANB1 zranb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZRANB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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