Western Blot (WB)
(Western Blot analysis of ENO1 expression in transfected 293T cell line by ENO1 polyclonal antibody. Lane 1: ENO1 transfected lysate (47.2kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human Enolase 1 Polyclonal Antibody | anti-ENO1 antibody
Enolase 1 (ENO1, 2-phospho-D-glycerate Hydro-lyase, alpha Enolase, C-myc Promoter-binding Protein, ENO1L1, MBP-1, MBPB1, MPB1, Non-neural Enolase, NNE, Phosphopyruvate Hydratase, PPH, Plasminogen-binding Protein) (HRP)
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Enolase 1; Polyclonal Antibody; Enolase 1 (ENO1; 2-phospho-D-glycerate Hydro-lyase; alpha Enolase; C-myc Promoter-binding Protein; ENO1L1; MBP-1; MBPB1; MPB1; Non-neural Enolase; NNE; Phosphopyruvate Hydratase; PPH; Plasminogen-binding Protein) (HRP); anti-ENO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ENO1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-ENO1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human ENO1, aa1-434 (NP_001419.1).
Immunogen Sequence
MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of ENO1 expression in transfected 293T cell line by ENO1 polyclonal antibody. Lane 1: ENO1 transfected lysate (47.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ENO1 expression in transfected 293T cell line by ENO1 polyclonal antibody. Lane 1: ENO1 transfected lysate (47.2kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-ENO1 antibody
Enolase 1 gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
Product Categories/Family for anti-ENO1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,928 Da
NCBI Official Full Name
alpha-enolase isoform 1
NCBI Official Synonym Full Names
enolase 1, (alpha)
NCBI Official Symbol
ENO1
NCBI Official Synonym Symbols
NNE; PPH; MPB1; ENO1L1
NCBI Protein Information
alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase
UniProt Protein Name
Alpha-enolase
Protein Family
UniProt Gene Name
ENO1
UniProt Synonym Gene Names
ENO1L1; MBPB1; MPB1; NNE
UniProt Entry Name
ENOA_HUMAN
NCBI Description
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Research Articles on ENO1
Similar Products
Product Notes
The ENO1 eno1 (Catalog #AAA6377386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Enolase 1 (ENO1, 2-phospho-D-glycerate Hydro-lyase, alpha Enolase, C-myc Promoter-binding Protein, ENO1L1, MBP-1, MBPB1, MPB1, Non-neural Enolase, NNE, Phosphopyruvate Hydratase, PPH, Plasminogen-binding Protein) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Enolase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ENO1 eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Enolase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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