Rabbit IRAK3 / IRAKM / IRAK-M Polyclonal Antibody | anti-IRAK3 antibody

Rabbit Polyclonal to Human IRAK3 / IRAKM / IRAK-M

Cat. Num. AAA241286

• Gene Names: IRAK3; ASRT5; IRAKM
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: IRAK3 / IRAKM / IRAK-M; Polyclonal Antibody; Rabbit Polyclonal to Human IRAK3 / IRAKM / IRAK-M; Anti-IRAK3 / IRAKM / IRAK-M Antibody (C-Terminus) IHC-plus; IRAK3; ASRT5; IRAK M; IRAK-3; IL1RAK M; IRAK-M; IRAKM; Human IRAK3; anti-IRAK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: peptide corresponding to amino acids from the carboxy terminus of human IRAK-M
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 596

• Applicable Applications for anti-IRAK3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: A peptide corresponding to amino acids from the carboxy terminus of human IRAK-M.
• Immunogen Type: Synthetic peptide
• Immunogen: IRAK3 / IRAKM / IRAK-M antibody was raised against a peptide corresponding to amino acids from the carboxy terminus of human IRAK-M.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-IRAK3 / IRAKM antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of IRAK-M in Rat Liver cells with IRAK-M antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of IRAK-M in mouse spleen (M) and rat liver (R) tissue lysates with IRAK-M antibody at 1 ug/ml.)

Related Product Information for anti-IRAK3 antibody

IRAK-M, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK-M is immediately downstream of the MyD88 adapter protein and immediately upstream of TRAF6. At least three mRNA transcripts have been reported - an approximately 8-kb doublet and a 2.5-kb transcript that matched isolated cDNA. IRAK-M (-/-) mice have increased inflammatory responses to bacterial infection.

Product Categories/Family for anti-IRAK3 antibody

Family: Protein Kinase; Subfamily: IRAK (Pelle)

NCBI and Uniprot Product Information

• NCBI GI #: 216547519
• NCBI GeneID: 11213
• NCBI Accession #: NP_009130.2
• NCBI GenBank Nucleotide #: NM_007199.2
• UniProt Accession #: Q9Y616; B4DQ57
• Molecular Weight: 60,608 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 3 isoform a
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 3
• NCBI Official Symbol: IRAK3
• NCBI Official Synonym Symbols: ASRT5; IRAKM
• NCBI Protein Information: interleukin-1 receptor-associated kinase 3; IRAK3; IL-1 receptor-associated kinase M
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 3
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK3
• UniProt Synonym Gene Names: IRAK-3; IRAK-M
• UniProt Entry Name: IRAK3_HUMAN

NCBI Description

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

IRAK3: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, TKL; Kinase, protein; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: interleukin-1 receptor complex; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; ATP binding

Biological Process: negative regulation of MAP kinase activity; regulation of protein complex disassembly; negative regulation of interleukin-12 production; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; response to virus; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; response to exogenous dsRNA; inhibition of NF-kappaB transcription factor; negative regulation of innate immune response; response to peptidoglycan; negative regulation of toll-like receptor signaling pathway; negative regulation of interleukin-6 production; toll-like receptor signaling pathway; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of protein catabolic process; negative regulation of protein complex disassembly

Disease: Asthma-related Traits, Susceptibility To, 5