Chromatin Immunoprecipitation (ChIP)
(HDAC4 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify HDAC4 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Rabbit anti-Human, Mouse HDAC4 Polyclonal Antibody | anti-HDAC4 antibody
HDAC4 antibody (pAb)
ChIP: 5-10ug per ChIP
ChIP-Seq: 5-10ug each WB: 1-2ug/ml dilution ChIP-Seq validation was performed's Epigenetics Services. For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Chromatin Immunoprecipitation (ChIP)
(HDAC4 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify HDAC4 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Chromatin Immunoprecipitation (ChIP)
(HDAC4 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify HDAC4 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Western Blot (WB)
(HDAC4 antibody (pAb) tested by Western blot. Detection of HDAC4 by Western blot. The analysis was performed using 293 nuclear extract and HDAC4 pAb. A protein band of approximate molecular weight of 140 kDa was detected.)
Western Blot (WB)
(HDAC4 antibody (pAb) tested by Western blot. Detection of HDAC4 by Western blot. The analysis was performed using 293 nuclear extract and HDAC4 pAb. A protein band of approximate molecular weight of 140 kDa was detected.)
Background: HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.
NCBI and Uniprot Product Information
Uniprot Description
HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Protein type: Nuclear receptor co-regulator; Hydrolase; Deacetylase; EC 3.5.1.98
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: nucleoplasm; transcriptional repressor complex; cytoplasm; histone deacetylase complex; actomyosin; neuromuscular junction; Z disc; cytosol; nucleus; A band
Molecular Function: potassium ion binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; transcription activator binding; NAD-dependent histone deacetylase activity (H3-K14 specific); zinc ion binding; sequence-specific DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; protein kinase binding; transcription factor binding; transcription corepressor activity
Biological Process: cardiac muscle hypertrophy; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; response to denervation involved in regulation of muscle adaptation; regulation of gene expression, epigenetic; negative regulation of cell proliferation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; negative regulation of glycolysis; inflammatory response; skeletal development; response to drug; nervous system development; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; negative regulation of transcription factor activity; histone deacetylation; osteoblast development; chromatin remodeling; positive regulation of protein sumoylation; regulation of protein binding; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent
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Product Notes
The HDAC4 hdac4 (Catalog #AAA388706) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HDAC4 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC4 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB). Applications Validated: ChIP: 5-10ug per ChIP ChIP-Seq: 5-10ug each WB: 1-2ug/ml dilution ChIP-Seq validation was performed's Epigenetics Services. For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the HDAC4 hdac4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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