Western Blot (WB)
(HDAC11 pAb tested by Western blot. Detection of HDAC11 by Western blot. The analysis was performed using HDAC11 pAb at a dilution of 2 ug/ml and brain whole-cell extracts from human (lane 1), mouse (lane 2) or rat origin.)
Mouse HDAC11 Polyclonal Antibody | anti-HDAC11 antibody
HDAC11 antibody (pAb)
Gene Names
HDAC11; HD11
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
HDAC11; Polyclonal Antibody; HDAC11 antibody (pAb); HD11; anti-HDAC11 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-HDAC11 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-3ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-3ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This HDAC11 antibody was raised against a peptide corresponding to amino acid residues 238-251 of human HDAC11.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(HDAC11 pAb tested by Western blot. Detection of HDAC11 by Western blot. The analysis was performed using HDAC11 pAb at a dilution of 2 ug/ml and brain whole-cell extracts from human (lane 1), mouse (lane 2) or rat origin.)
Western Blot (WB)
(HDAC11 pAb tested by Western blot. Detection of HDAC11 by Western blot. The analysis was performed using HDAC11 pAb at a dilution of 2 ug/ml and brain whole-cell extracts from human (lane 1), mouse (lane 2) or rat origin.)
Related Product Information for anti-HDAC11 antibody
Short Description: Our HDAC11 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human, Mouse and Rat samples.
Background: HDAC11 (Histone Deacetylase 11) is a member of the class IV mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). In humans, expression of HDAC11 is limited to only a few organs, including brain, heart, muscle and kidney, suggesting an important role of HDAC11 in those tissues.
Background: HDAC11 (Histone Deacetylase 11) is a member of the class IV mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). In humans, expression of HDAC11 is limited to only a few organs, including brain, heart, muscle and kidney, suggesting an important role of HDAC11 in those tissues.
Product Categories/Family for anti-HDAC11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,124 Da
NCBI Official Full Name
histone deacetylase 11 isoform 2
NCBI Official Synonym Full Names
histone deacetylase 11
NCBI Official Symbol
HDAC11
NCBI Official Synonym Symbols
HD11
NCBI Protein Information
histone deacetylase 11
UniProt Protein Name
Histone deacetylase 11
Protein Family
UniProt Gene Name
HDAC11
UniProt Synonym Gene Names
HD11
Uniprot Description
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Similar Products
Product Notes
The HDAC11 hdac11 (Catalog #AAA388406) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HDAC11 antibody (pAb) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-3ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the HDAC11 hdac11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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