Rabbit anti-Human TTK Polyclonal Antibody | anti-TTK antibody

TTK, NT (TTK, MPS1, MPS1L1, Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase) (Biotin)

Cat. Num. AAA6360015

• Gene Names: TTK; ESK; PYT; CT96; MPH1; MPS1; MPS1L1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TTK; Polyclonal Antibody; NT (TTK; MPS1; MPS1L1; Dual specificity protein kinase TTK; Phosphotyrosine picked threonine-protein kinase) (Biotin); Phosphotyrosine picked threonine-protein kinase; anti-TTK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TTK antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of human TTK.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TTK Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the TTK antibody detected the TTK protein (arrow).)

Related Product Information for anti-TTK antibody

This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-TTK antibody

Antibodies; Protein Kinases

References

Cui, Y., et al. J. Biol. Chem. 285(43):32988-32998(2010). Maciejowski, J., et al. J. Cell Biol. 190(1):89-100(2010). Santaguida, S., et al. J. Cell Biol. 190(1):73-87(2010). Hewitt, L., et al. J. Cell Biol. 190(1):25-34(2010). Lan, W., et al. J. Cell Biol. 190(1):21-24(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 23308722
• NCBI GeneID: 7272
• NCBI Accession #: NP_003309.2
• NCBI GenBank Nucleotide #: NM_003318.4
• UniProt Accession #: P33981; Q15272; Q5TCS0; Q9BW51; Q9NTM0; A8K8U5; B2RDW2; E1P543
• Molecular Weight: 96,944 Da
• NCBI Official Full Name: dual specificity protein kinase TTK isoform 1
• NCBI Official Synonym Full Names: TTK protein kinase
• NCBI Official Symbol: TTK
• NCBI Official Synonym Symbols: ESK; PYT; CT96; MPH1; MPS1; MPS1L1
• NCBI Protein Information: dual specificity protein kinase TTK
• UniProt Protein Name: Dual specificity protein kinase TTK
• Protein Family: Protein tramtrack
• UniProt Gene Name: TTK
• UniProt Synonym Gene Names: MPS1; MPS1L1; PYT
• UniProt Entry Name: TTK_HUMAN

NCBI Description

This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]

Uniprot Description

TTK: a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint.

Protein type: Kinase, protein; EC 2.7.12.1; Protein kinase, Other; Cancer Testis Antigen (CTA); Protein kinase, dual-specificity (non-receptor); Other group; TTK family

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: membrane; spindle

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; mitotic spindle organization and biogenesis; mitotic cell cycle spindle assembly checkpoint; positive regulation of cell proliferation; spindle organization and biogenesis; chromosome separation