SDS-Page
(Detection of Fibromodulin with MBS619893. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 0.3ug/ml sheep anti-Fibromodulin.)
Sheep Fibromodulin Polyclonal Antibody | anti-FMOD antibody
Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein)
Gene Names
FMOD; SLRR2E; FMOD
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Fibromodulin; Polyclonal Antibody; Fibromodulin (FMOD; FM; Collagen-binding 59kD Protein); Anti -Fibromodulin (FMOD; anti-FMOD antibody
Host
Sheep
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Fibromodulin. Species Crossreactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-FMOD antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 0.3ug/ml
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 0.3ug/ml
Immunogen
NS0-derived recombinant human Fibromodulin (aa75-376).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of Fibromodulin with MBS619893. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 0.3ug/ml sheep anti-Fibromodulin.)
SDS-Page
(Detection of Fibromodulin with MBS619893. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 0.3ug/ml sheep anti-Fibromodulin.)
Related Product Information for anti-FMOD antibody
Fibromodulin (FMOD; also FM and collagen-binding 59kD protein) is a secreted, 60-70kD class II member of the SLRP family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-B. Fibromodulin is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers. Mature human Fibromodulin is 358aa in length (aa19-376). It contains 12 LRRs (Leu-rich repeats) (aa98-376), with the seventh and eleventh LRR principally responsible for collagen type I binding. Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation. There is one splice form that shows a deletion of aa88-101. Over aa76-376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin.
Product Categories/Family for anti-FMOD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
43,179 Da
NCBI Official Full Name
fibromodulin
NCBI Official Synonym Full Names
fibromodulin
NCBI Official Symbol
FMOD
NCBI Official Synonym Symbols
SLRR2E; FMOD
NCBI Protein Information
fibromodulin; FM; KSPG fibromodulin; fibromodulin proteoglycan; collagen-binding 59 kDa protein; keratan sulfate proteoglycan fibromodulin
UniProt Protein Name
Fibromodulin
Protein Family
UniProt Gene Name
FMOD
UniProt Synonym Gene Names
FM; SLRR2E
UniProt Entry Name
FMOD_HUMAN
NCBI Description
Fibromodulin is a member of a family of small interstitial proteoglycans, containing a central region composed of leucine-rich repeats with 4 keratan sulfate chains flanked by disulfide-bonded terminal domains. It may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. [provided by RefSeq]
Uniprot Description
FMOD: Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region
Biological Process: keratan sulfate metabolic process; transforming growth factor beta receptor complex assembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; pathogenesis; keratan sulfate catabolic process
Research Articles on FMOD
1. study shows (1) a detailed description of ectopic ossification (EO) formed by Bgn, Fmod or combined depletion, (2) the role of exercise in modulating EO and (3) that Bgn and Fmod are critical in controlling motor function.
2. tyrosine sulfate-rich domains of the LRR proteins fibromodulin and osteoadherin bind motifs of basic clusters in a variety of heparin-binding proteins, including bioactive factors
3. differentially expressed in mesenchymal stromal cell subset with reduced differentiation potential
4. Observational study of gene-disease association. (HuGE Navigator)
5. Ultraviolet B and A irradiation induce FMOD expression in fibroblasts in vitro.
6. Homologous sequence in lumican and fibromodulin leucine-rich repeat 5-7 competes for collagen binding.
7. CD200 blockade was further shown to stimulate antigen-specific T cell responses towards the CLL-associated antigen fibromodulin
8. Specific decorin and fibromodulin core protein fragments in degenerate meniscus and/or human articular cartilage may be of value as biomarkers of disease.
9. Fibromodulin gene is constantly expressed in human epidermal keratinocytes.
10. The collagen-binding Glu-353 and Lys-355 residues in fibromodulin are exposed on the exterior of the beta-sheet-loop structure of the leucine-rich repeat, which resembles the location of interacting residues in other leucine-rich repeat proteins.
2. tyrosine sulfate-rich domains of the LRR proteins fibromodulin and osteoadherin bind motifs of basic clusters in a variety of heparin-binding proteins, including bioactive factors
3. differentially expressed in mesenchymal stromal cell subset with reduced differentiation potential
4. Observational study of gene-disease association. (HuGE Navigator)
5. Ultraviolet B and A irradiation induce FMOD expression in fibroblasts in vitro.
6. Homologous sequence in lumican and fibromodulin leucine-rich repeat 5-7 competes for collagen binding.
7. CD200 blockade was further shown to stimulate antigen-specific T cell responses towards the CLL-associated antigen fibromodulin
8. Specific decorin and fibromodulin core protein fragments in degenerate meniscus and/or human articular cartilage may be of value as biomarkers of disease.
9. Fibromodulin gene is constantly expressed in human epidermal keratinocytes.
10. The collagen-binding Glu-353 and Lys-355 residues in fibromodulin are exposed on the exterior of the beta-sheet-loop structure of the leucine-rich repeat, which resembles the location of interacting residues in other leucine-rich repeat proteins.
Similar Products
Product Notes
The FMOD fmod (Catalog #AAA619893) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fibromodulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 0.3ug/ml. Researchers should empirically determine the suitability of the FMOD fmod for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibromodulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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