Western Blot (WB)
((0.3ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Glycine receptor, alpha 1 Polyclonal Antibody | anti-Glra1 antibody
Goat anti-Glycine receptor, alpha 1 (intracellular) Antibody
Gene Names
Glra1; GLYRA1
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Glycine receptor; alpha 1; Polyclonal Antibody; Goat anti-Glycine receptor; alpha 1 (intracellular) Antibody; Glra1; glycine receptor; GLYRA1; glycine receptor 48 kDa subunit; glycine receptor strychnine-binding subunit; glycine receptor subunit alpha-1; alpha 1 subunit; Glra1 antibody; glycine receptor 48 kDa subunit antibody; glycine receptor strychnine-binding subunit antibody; glycine receptor subunit alpha-1 antibody; alpha 1 antibody; alpha 1 subunit antibody; GLYRA1 antibody; alpha 1 (intracellular); anti-Glra1 antibody
Host
Goat
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CLQAKDGISVKGAN
Sequence Length
449
Applicable Applications for anti-Glra1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 60kDa band observed in Mouse and Rat Testis lysates (calculated MW of 51.7kDa according to Mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 60kDa band observed in Mouse and Rat Testis lysates (calculated MW of 51.7kDa according to Mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence CLQAKDGISVKGAN, from the internal region of the protein sequence according to NP_037265.1.
Epitope
Internal region
Note
The immunizing peptide represents part of the cytoplasmic domain. The antibody is not expected to cross-react with other glycine receptor alpha proteins.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,686 Da
NCBI Official Full Name
glycine receptor subunit alpha-1
NCBI Official Synonym Full Names
glycine receptor, alpha 1
NCBI Official Symbol
Glra1
NCBI Official Synonym Symbols
GLYRA1
NCBI Protein Information
glycine receptor subunit alpha-1
UniProt Protein Name
Glycine receptor subunit alpha-1
Protein Family
UniProt Gene Name
Glra1
UniProt Synonym Gene Names
Glyr
UniProt Entry Name
GLRA1_RAT
NCBI Description
glycine receptor strychnine binding subunit; generates glycine gated strychnine sensitive chloride current in the central nervous system [RGD, Feb 2006]
Uniprot Description
Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:1716350, PubMed:11981023, PubMed:2483325, PubMed:3037383). Channel opening is also triggered by taurine and beta-alanine (PubMed:1716350). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (). Potentiation of channel activity by intoxicating levels ot ethanol contribute to the sedative effects of ethanol ().
Research Articles on Glra1
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Product Notes
The Glra1 glra1 (Catalog #AAA422956) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Glycine receptor, alpha 1 (intracellular) Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Glycine receptor, alpha 1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 60kDa band observed in Mouse and Rat Testis lysates (calculated MW of 51.7kDa according to Mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the Glra1 glra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CLQAKDGISV KGAN. It is sometimes possible for the material contained within the vial of "Glycine receptor, alpha 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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