Western Blot (WB)
((0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat IL-17D Polyclonal Antibody | anti-IL17D antibody
Goat anti-IL-17D Antibody
Gene Names
IL17D; IL27; IL-27; IL-17D
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
IL-17D; Polyclonal Antibody; Goat anti-IL-17D Antibody; Goat Anti-IL-17D (aa55-65) Antibody; IL17D; interleukin 17D; IL-22; IL-27; IL27; interleukin 27; interleukin-17D; interleukin-27; IL17D antibody; interleukin 17D antibody; IL-17D antibody; IL-22 antibody; IL-27 antibody; IL27 antibody; interleukin 27 antibody; interleukin-17D antibody; interleukin-27 antibody; anti-IL17D antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EKDADSINSSIDK
Sequence Length
202
Applicable Applications for anti-IL17D antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 28kDa band observed in Human Adipose lysates (calculated MW of 21.9kDa according to NP_612141.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 28kDa band observed in Human Adipose lysates (calculated MW of 21.9kDa according to NP_612141.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-EKDADSINSSIDK, from the internal region (near C Terminus) of the protein sequence according to NP_612141.1.
Epitope
Internal region (near C Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,893 Da
NCBI Official Full Name
interleukin-17D
NCBI Official Synonym Full Names
interleukin 17D
NCBI Official Symbol
IL17D
NCBI Official Synonym Symbols
IL27; IL-27; IL-17D
NCBI Protein Information
interleukin-17D
UniProt Protein Name
Interleukin-17D
Protein Family
UniProt Gene Name
IL17D
UniProt Synonym Gene Names
IL27; IL-17D; IL-27
UniProt Entry Name
IL17D_HUMAN
NCBI Description
The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent. [provided by RefSeq, Jul 2008]
Uniprot Description
IL17D: Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells. Belongs to the IL-17 family.
Protein type: Secreted, signal peptide; Secreted; Cytokine
Chromosomal Location of Human Ortholog: 13q11
Cellular Component: extracellular space
Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding
Biological Process: cell surface receptor linked signal transduction; inflammatory response
Research Articles on IL17D
Similar Products
Product Notes
The IL17D il17d (Catalog #AAA423555) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IL-17D Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-17D can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 28kDa band observed in Human Adipose lysates (calculated MW of 21.9kDa according to NP_612141.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the IL17D il17d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EKDADSINSS IDK. It is sometimes possible for the material contained within the vial of "IL-17D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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