Goat STAT5A Polyclonal Antibody | anti-STAT5A antibody

Goat anti-STAT5A Antibody

Cat. Num. AAA422892

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: STAT5A; Polyclonal Antibody; Goat anti-STAT5A Antibody; Goat Anti-STAT5A (aa681-692) Antibody; signal transducer and activator of transcription 5A; MGF; STAT5; MGF antibody; signal transducer and activator of transcription 5A antibody; STAT5 antibody; STAT5A antibody; anti-STAT5A antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DSRLSPPAGLFTSAR
• Sequence Length: 794

• Applicable Applications for anti-STAT5A antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 100kDa band observed in lysates of cell lines K562, Molt4 and NIH3T3 (calculated MW of 90.6kDa according to NP_003143.2). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-DSRLSPPAGLFTSAR, from the C Terminus of the protein sequence according to NP_003143.2.
• Epitope: C Terminus
• Note: This antibody is not expected to cross-react with STAT5B.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of K562 (A), Molt4 (B) and NIH3T3 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 21618342
• NCBI GeneID: 6776
• NCBI Accession #: NP_003143.2
• NCBI GenBank Nucleotide #: NM_003152.3
• Molecular Weight: 86,949 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Uniprot Description

STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: DNA binding; protein binding; protein-tyrosine kinase activity; signal transducer activity; transcription factor activity

Biological Process: 2-oxoglutarate metabolic process; allantoin metabolic process; citrate metabolic process; creatine metabolic process; creatinine metabolic process; fatty acid metabolic process; isoleucine metabolic process; JAK-STAT cascade; lactation; oxaloacetate metabolic process; peptidyl-tyrosine phosphorylation; positive regulation of blood vessel endothelial cell migration; positive regulation of endothelial cell proliferation; regulation of multicellular organism growth; regulation of transcription from RNA polymerase II promoter; succinate metabolic process; taurine metabolic process; transcription, DNA-dependent; valine metabolic process