RAB17 recombinant protein

RAB17, 1-212aa, Human, His tag, E Coli

Cat. Num. AAA204663

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RAB17; 1-212aa; Human; His tag; E Coli; Ras-related protein Rab-17; FLJ12538; RAB17 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAQAHRTPQP RAAPSQPRVF KLVLLGSGSV GKSSLALRYV KNDFKSILPT VGCAFFTKVV DVGATSLKLE IWDTAGQEKY HSVCHLYFRG ANAALLVYDI TRKDSFLKAQ QWLKDLEEEL HPGEVLVMLV GNKTDLSQER EVTFQEGKEF ADSQKLLFME TSAKLNHQVS EVFNTVAQEL LQRSDEEGQA LRGDAAVALN KGPARQAKCC AH
• Sequence Length: 212

• Applicable Applications for RAB17 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RAB17 recombinant protein

RAB17, also known as Ras-related protein Rab17, belongs to the small GTPase superfamily, Rab GTPases are localized to various intracellular compartments and are known to play important regulatory roles in membrane trafficking. It is regulated traffic through the apical recycling endosome, suggested function in polarized sorting in epithelial cells. Recombinant human RAB17 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for RAB17 recombinant protein

Signal Transduction

References

Hunziker W. et al. (1998) J Biol Chem. 273:15734-41; Zacchi P. et al. (1998) J Cell Biol. 140:1039-53

NCBI and Uniprot Product Information

• NCBI GI #: 11967981
• NCBI GeneID: 64284
• NCBI Accession #: NP_071894
• NCBI GenBank Nucleotide #: NM_022449.3
• UniProt Accession #: Q9H0T7; Q53QV6; Q6IA73; Q6PJZ0; Q9BVU1; Q9H9U9
• Molecular Weight: 25.6 kDa (232aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ras-related protein Rab-17
• NCBI Official Synonym Full Names: RAB17, member RAS oncogene family
• NCBI Official Symbol: RAB17
• NCBI Protein Information: ras-related protein Rab-17
• UniProt Protein Name: Ras-related protein Rab-17
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB17

NCBI Description

The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase (Lutcke et al., 1993 [PubMed 8486736]).[supplied by OMIM, Oct 2009]

Uniprot Description

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. Mainly observed in epithelial cells, transcytosis mediates for instance, the transcellular transport of immunoglobulins from the basolateral surface to the apical surface. Most probably controls membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis. Required for melanosome transport and release from melanocytes, it also regulates dendrite and dendritic spine development (). May also play a role in cell migration.