Goat NNMT Polyclonal Antibody | anti-NNMT antibody

Goat anti-NNMT (aa171-182) Antibody

Cat. Num. AAA423186

• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: NNMT; Polyclonal Antibody; Goat anti-NNMT (aa171-182) Antibody; Goat Anti-NNMT Antibody; nicotinamide N-methyltransferase; NNMT antibody; nicotinamide N-methyltransferase antibody; NNMT (aa171-182); anti-NNMT antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PDLPTYCRALRN
• Sequence Length: 264

• Applicable Applications for anti-NNMT antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 28kDa band observed in lysates of cell line HeLa (calculated MW of 29.6kDa according to NP_006160.1). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence PDLPTYCRALRN, from the internal region of the protein sequence according to NP_006160.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 5453790
• NCBI GeneID: 4837
• NCBI Accession #: NP_006160.1
• NCBI GenBank Nucleotide #: NM_006169.2
• Molecular Weight: 29,574 Da
• NCBI Official Full Name: nicotinamide N-methyltransferase
• NCBI Official Synonym Full Names: nicotinamide N-methyltransferase
• NCBI Official Symbol: NNMT
• NCBI Protein Information: nicotinamide N-methyltransferase
• UniProt Protein Name: Nicotinamide N-methyltransferase
• UniProt Gene Name: NNMT
• UniProt Entry Name: NNMT_HUMAN

NCBI Description

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

Uniprot Description

NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.1.1.1; Methyltransferase

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function: nicotinamide N-methyltransferase activity; pyridine N-methyltransferase activity

Biological Process: methylation; organ regeneration; response to drug; response to organic nitrogen