Western Blot (WB)
((0.3ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat GDE1/MIR16 Polyclonal Antibody | anti-GDE1 antibody
Goat anti-GDE1/MIR16 (aa230-242) Antibody
Gene Names
GDE1; MIR16; 363E6.2
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GDE1/MIR16; Polyclonal Antibody; Goat anti-GDE1/MIR16 (aa230-242) Antibody; GDE1; glycerophosphodiester phosphodiesterase 1; 363E6.2; MIR16; RGS16-interacting membrane protein; membrane interacting protein of RGS16; membrane-interacting protein of RGS16; GDE1 antibody; glycerophosphodiester phosphodiesterase 1 antibody; 363E6.2 antibody; MIR16 antibody; RGS16-interacting membrane protein antibody; membrane interacting protein of RGS16 antibody; membrane-interacting protein of RGS16 antibody; GDE1 / MIR16 (aa230-242); anti-GDE1 antibody
Host
Goat
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SLSHTGDGKPRYD
Sequence Length
331
Applicable Applications for anti-GDE1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 50kDa band observed in Mouse and Rat Brain lysates (calculated MW of 37.7kDa according to NP_057725.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 50kDa band observed in Mouse and Rat Brain lysates (calculated MW of 37.7kDa according to NP_057725.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-SLSHTGDGKPRYD, from the internal region of the protein sequence according to NP_057725.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,718 Da
NCBI Official Full Name
glycerophosphodiester phosphodiesterase 1 isoform 1
NCBI Official Synonym Full Names
glycerophosphodiester phosphodiesterase 1
NCBI Official Symbol
GDE1
NCBI Official Synonym Symbols
MIR16; 363E6.2
NCBI Protein Information
glycerophosphodiester phosphodiesterase 1
UniProt Protein Name
Glycerophosphodiester phosphodiesterase 1
Protein Family
UniProt Gene Name
GDE1
UniProt Synonym Gene Names
MIR16
UniProt Entry Name
GDE1_HUMAN
Uniprot Description
GDE1: Has glycerophosphoinositol phosphodiesterase activity. Has little or no activity towards glycerophosphocholine. GDE1 activity can be modulated by G-protein signaling pathways. Belongs to the glycerophosphoryl diester phosphodiesterase family.
Protein type: Phosphodiesterase; EC 3.1.4.44; Membrane protein, multi-pass; Lipid Metabolism - glycerophospholipid; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16p12-p11.2
Cellular Component: cytoplasmic vesicle membrane; integral to membrane; plasma membrane
Molecular Function: glycerophosphodiester phosphodiesterase activity; glycerophosphoinositol glycerophosphodiesterase activity; metal ion binding
Biological Process: glycerophospholipid catabolic process; lipid metabolic process
Research Articles on GDE1
Similar Products
Product Notes
The GDE1 gde1 (Catalog #AAA423527) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GDE1/MIR16 (aa230-242) Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's GDE1/MIR16 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 50kDa band observed in Mouse and Rat Brain lysates (calculated MW of 37.7kDa according to NP_057725.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the GDE1 gde1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SLSHTGDGKP RYD. It is sometimes possible for the material contained within the vial of "GDE1/MIR16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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