Western Blot (WB)
(Western Blot analysis of GIYD2 expression in transfected 293T cell line by GIYD2 polyclonal antibody. Lane 1: GIYD2 transfected lysate (30.25kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human GIYD2 Polyclonal Antibody
GIYD2 (Structure-specific Endonuclease Subunit SLX1, GIY-YIG Domain-containing Protein 1, SLX1A, GIYD1, SLX1, SLX1B, SLX1, FLJ23439, MGC2532, MGC5178)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
GIYD2; Polyclonal Antibody; GIYD2 (Structure-specific Endonuclease Subunit SLX1; GIY-YIG Domain-containing Protein 1; SLX1A; GIYD1; SLX1; SLX1B; FLJ23439; MGC2532; MGC5178); Anti -GIYD2 (Structure-specific Endonuclease Subunit SLX1; anti-GIYD2 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GIYD2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MGPAGVAARPGRFFGVYLLYCLNPRYRGRVYVGFTVNTARRVQQHNGGRKKGGAWRTSGRGPWEMVLVVHGFPSSVAALRFEWAWQHPHASRRLAHVGPRLRGETAFAFHLRVLAHMLRAPPWARLPLTLRWVRPDLRQDLCLPPPPHVPLAFGPPPPQAPAPRRRAGPFDDAEPEPDQGDPGACCSLCAQTIQDEEGPLCCPHPGCLLRAHVICLAEEFLQEEPGQLLPLEGQCPCCEKSLLWGDLIWLCQMDTEKEVEDSELEEAHWTDLLET
Applicable Applications for anti-GIYD2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human GIYD2, aa1-275 (NP_076949.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of GIYD2 expression in transfected 293T cell line by GIYD2 polyclonal antibody. Lane 1: GIYD2 transfected lysate (30.25kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of GIYD2 expression in transfected 293T cell line by GIYD2 polyclonal antibody. Lane 1: GIYD2 transfected lysate (30.25kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-GIYD2 antibody
Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products.
Product Categories/Family for anti-GIYD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
GIY-YIG domain containing 2
Uniprot Description
Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products.
Similar Products
Product Notes
The GIYD2 (Catalog #AAA6003575) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GIYD2 (Structure-specific Endonuclease Subunit SLX1, GIY-YIG Domain-containing Protein 1, SLX1A, GIYD1, SLX1, SLX1B, SLX1, FLJ23439, MGC2532, MGC5178) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GIYD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the GIYD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGPAGVAARP GRFFGVYLLY CLNPRYRGRV YVGFTVNTAR RVQQHNGGRK KGGAWRTSGR GPWEMVLVVH GFPSSVAALR FEWAWQHPHA SRRLAHVGPR LRGETAFAFH LRVLAHMLRA PPWARLPLTL RWVRPDLRQD LCLPPPPHVP LAFGPPPPQA PAPRRRAGPF DDAEPEPDQG DPGACCSLCA QTIQDEEGPL CCPHPGCLLR AHVICLAEEF LQEEPGQLLP LEGQCPCCEK SLLWGDLIWL CQMDTEKEVE DSELEEAHWT DLLET. It is sometimes possible for the material contained within the vial of "GIYD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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