Western Blot (WB)
(Western Blot analysis of NEIL2 expression in transfected 293T cell line by NEIL2 polyclonal antibody. Lane 1: NEIL2 transfected lysate (36.8kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human NEIL2 Polyclonal Antibody | anti-NEIL2 antibody
NEIL2 (Endonuclease 8-like 2, DNA Glycosylase/AP Lyase Neil2, DNA-(apurinic or apyrimidinic site) Lyase Neil2, Endonuclease VIII-like 2, Nei Homolog 2, Nei-like Protein 2, FLJ31644, MGC2832, MGC4505, NEH2, NEI2)
Serum
Serum
Western Blot (WB)
(Western Blot analysis of NEIL2 expression in transfected 293T cell line by NEIL2 polyclonal antibody. Lane 1: NEIL2 transfected lysate (36.8kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of NEIL2 expression in transfected 293T cell line by NEIL2 polyclonal antibody. Lane 1: NEIL2 transfected lysate (36.8kD). Lane 2: Non-transfected lysate.)
Immunoprecipitation (IP)
(Immunoprecipitation of NEIL2 transfected lysate using NEIL2 rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEIL2 mouse polyclonal antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation of NEIL2 transfected lysate using NEIL2 rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEIL2 mouse polyclonal antibody.)
NCBI and Uniprot Product Information
NCBI Description
NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226])[supplied by OMIM, Mar 2008]
Uniprot Description
Function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Ref.7 Ref.8 Ref.9 Ref.10
Catalytic activity: Removes damaged bases from DNA, leaving an abasic site.The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Enzyme regulation: Acetylation of Lys-50 leads to loss of DNA nicking activity. Acetylation of Lys-154 has no effect.
Subunit structure: Binds EP300.
Subcellular location: Nucleus Ref.7.
Tissue specificity: Detected in testis, skeletal muscle, heart, brain, placenta, lung, pancreas, kidney and liver. Ref.7
Domain: The zinc-finger domain is important for DNA binding.
Sequence similarities: Belongs to the FPG family.Contains 1 FPG-type zinc finger.
Research Articles on NEIL2
Similar Products
Product Notes
The NEIL2 neil2 (Catalog #AAA649764) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEIL2 (Endonuclease 8-like 2, DNA Glycosylase/AP Lyase Neil2, DNA-(apurinic or apyrimidinic site) Lyase Neil2, Endonuclease VIII-like 2, Nei Homolog 2, Nei-like Protein 2, FLJ31644, MGC2832, MGC4505, NEH2, NEI2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEIL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Researchers should empirically determine the suitability of the NEIL2 neil2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPEGPLVRKF HHLVSPFVGQ QVVKTGGSSK KLQPASLQSL WLQDTQVHGK KLFLRFDLDE EMGPPGSSPT PEPPQKEVQK EGAADPKQVG EPSGQKTLDG SSRSAELVPQ GEDDSEYLER DAPAGDAGRW LRVSFGLFGS VWVNDFSRAK KANKRGDWRD PSPRLVLHFG GGGFLAFYNC QLSWSSSPVV TPTCDILSEK FHRGQALEAL GQAQPVCYTL LDQRYFSGLG NIIKNEALYR AGIHPLSLGS VLSASRREVL VDHVVEFSTA WLQGKFQGRP QHTQVYQKEQ CPAGHQVMKE AFGPEDGLQR LTWWCPQCQP QLSEEPEQCQ FS. It is sometimes possible for the material contained within the vial of "NEIL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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