Western Blot (WB)
(Western Blot analysis of BTBD12 expression in transfected 293T cell line by MBS6012428. Lane 1: BTBD12 transfected lysate (126.61kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human SLX4 Polyclonal Antibody | anti-SLX4 antibody
SLX4 (BTBD12, Structure-specific Endonuclease Subunit SLX4, BTB/POZ Domain-containing Protein 12, KIAA1784, KIAA1987)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of BTBD12 expression in transfected 293T cell line by MBS6012428. Lane 1: BTBD12 transfected lysate (126.61kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of BTBD12 expression in transfected 293T cell line by MBS6012428. Lane 1: BTBD12 transfected lysate (126.61kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a structure-specific endonuclease subunit. The encoded protein contains a central BTB domain and it forms a multiprotein complex with the ERCC4(XPF)-ERCC1, MUS81-EME1, and SLX1 endonucleases, and also associates with MSH2/MSH3 mismatch repair complex, telomere binding complex TERF2(TRF2)-TERF2IP(RAP1), the protein kinase PLK1 and the uncharacterized protein C20orf94. The multiprotein complex is required for repair of specific types of DNA lesions and is critical for cellular responses to replication fork failure. The encoded protein acts as a docking platform for the assembly of multiple structure-specific endonucleases.[provided by RefSeq, Jan 2011]
Uniprot Description
SLX4: regulatory subunit of different structure-specific endonucleases. Required for DNA recombination and repair. Together with SLX1 forms a Holliday junction (HJ) resolvase. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating HJs. Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. Required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks. Involved in Rad1/Rad10pdependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing. Interacts with MSH2; component of the MSH2-MSH3 mismatch repair complex. Interacts with TERF2-TERF2IP. Interacts with PLK1 and C20ORF94. Localizes to sites of DNA damage. Two alternatively spliced human isoforms have been described.
Protein type: DNA repair, damage; Deoxyribonuclease
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nucleoplasm; nuclear chromosome, telomeric region; nuclear chromatin; cytoplasm; cell junction
Molecular Function: protein binding; 5'-flap endonuclease activity; 3'-flap endonuclease activity; enzyme activator activity; crossover junction endodeoxyribonuclease activity
Biological Process: positive regulation of catalytic activity; nucleotide-excision repair; DNA replication; DNA repair; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination
Disease: Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group P
Research Articles on SLX4
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Product Notes
The SLX4 slx4 (Catalog #AAA6012428) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SLX4 (BTBD12, Structure-specific Endonuclease Subunit SLX4, BTB/POZ Domain-containing Protein 12, KIAA1784, KIAA1987) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLX4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the SLX4 slx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVNNPHLSDV QFQTDSGEVL YAHKFVLYAR CPLLIQYVNN EGFSAVEDGV LTQRVLLGDV STEAARTFLH YLYTADTGLP PGLSSELSSL AHRFGVSELV HLCEQVPIAT DSEGKPWEEK EAENCESRAE NFQELLRSMW ADEEEEAETL LKSKDHEEDQ ENVNEAEMEE IYEFAATQRK LLQEERAAGA GEDADWLEGG SPVSGQLLAG VQVQKQWDKV EEMEPLEPGR DEAATTWEKM GQCALPPPQG QHSGARGAEA PEQEAPEEAL GHSSCSSPSR DCQAERKEGS LPHSDDAGDY EQLFSSTQGE ISEPSQITSE PEEQSGAVRE RGLEVSHRLA PWQASPPHPC RFLLGPPQGG SPRGSHHTSG SSLSTPRSRG GTSQVGSPTL LSPAVPSKQK RDRSILTLSK EPGHQKGKER RSVLECRNKG VLMFPEKSLS IDLTQSNPDH SSSRSQKSSS KLNEEDEVIL LLDSDEELEL EQTKMKSISS DPLEEKKALE ISPRSCELFS IIDVDADQEP SQSPPRSEAV LQQEDEGALP ENRGSLGRRG APWLFCDRES SPSEASTTDT SWLVPATPLA SRSRDCSSQT QISSLRSGLA VQAVTQHTPR ASVGNREGNE VAQKFSVIRP QTPPPQTPSS CLTPVSPGTS DGRRQGHRSP SRPHPGGHPH SSPLAPHPIS GDRAHFSRRF LKHSPPGPSF LNQTPAGEVV EVGDSDDEQE VASHQANRSP PLDSDPPIPI DDCCWHMEPL SPIPIDHWNL ERTGPLSTSS PSRRMNEAAD SRDCRSPGLL DTTPIRGSCT TQRKLQEKSS GAGSLGNSRP SFLNSALWDV WDGEEQRPPE TPPPAQMPSA GGAQKPEGLE TPKGANRKKN LPPKVPITPM PQYSIMETPV LKKELDRFGV RPLPKRQMVL KLKEIFQYTH QTLDSDSEDE SQSSQPLLQA PHCQTLASQT YKPSRAGVHA QQEATTGPGA HRPKGPAKTK GPRHQRKHHE SITPPSRSPT KEAPPGLNDD AQIPASQESV ATSVDGSDSS LSSQSSSSCE FGAAFESAGE EEGEGEVSAS QAAVQAADTD EALRCYIRSK PALYQKVLLY QPFELRELQA ELRQNGLRVS SRRLLDFLDT HCITFTTAAT RREKLQGRRR QPRGKKKVER N. It is sometimes possible for the material contained within the vial of "SLX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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