Rabbit GEM Polyclonal Antibody | anti-GEM antibody

GEM Polyclonal Antibody

Cat. Num. AAA3015292

• Gene Names: GEM; KIR
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: GEM; Polyclonal Antibody; GEM Polyclonal Antibody; GTP binding mitogen induced T cell protein; GTP binding protein expressed in mitogen stimulated T cells; GTP binding protein GEM; GTP binding protein overexpressed in skeletal muscle; Kinase inducible Ras like protein; KIR; MGC26294; RAS like protein KIR; GEM_HUMAN.??; anti-GEM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse
• Clonality: Polyclonal
• Specificity: GEM Polyclonal Antibody detects endogenous levels of GEM protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-GEM antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human GEM:201-296/296
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GEM) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Related Product Information for anti-GEM antibody

Gem belongs to the Rad/Gem/Kir (RGK) subfamily of Ras-related GTPases, which lack typical C-terminal amino acid motifs for isoprenylation. Rad and Gem bind calmodulin in a Ca2+-dependent manner via this C-terminal extension, involving residues 278-297 in human Rad. High intracellular Gem levels, which interact with intact microtubules and microfilaments, promote profound changes in cell morphology. Ectopic Gem expression is sufficient to stimulate cell flattening and neurite extension in N1E-115 and SH-SY5Y neuroblastoma cells, suggesting a role for Gem in cytoskeletal rearrangement and/or morphological differentiation of neurons. Gem was also observed in developing trigeminal nerve ganglia in 12.5 day mouse embryos, demonstrating that Gem expression is a property of normal ganglionic development. The interaction of Gem with beta-subunits regulates Ca2+ channel expression at the cell surface. The human Gem gene maps to chromosome -8q22.1.

NCBI and Uniprot Product Information

• NCBI GI #: 32483371
• NCBI GeneID: 2669
• NCBI Accession #: NP_859053.1
• NCBI GenBank Nucleotide #: NM_181702.2
• UniProt Accession #: P55040; B2RA31
• Molecular Weight: 33,949 Da
• NCBI Official Full Name: GTP-binding protein GEM
• NCBI Official Synonym Full Names: GTP binding protein overexpressed in skeletal muscle
• NCBI Official Symbol: GEM
• NCBI Official Synonym Symbols: KIR
• NCBI Protein Information: GTP-binding protein GEM
• UniProt Protein Name: GTP-binding protein GEM
• Protein Family: Geminin
• UniProt Gene Name: GEM
• UniProt Synonym Gene Names: KIR

NCBI Description

The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

GEM: Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Belongs to the small GTPase superfamily. RGK family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, RGK

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: internal side of plasma membrane; midbody; spindle midzone

Molecular Function: GDP binding; GTP binding; GTPase activity; magnesium ion binding; protein binding

Biological Process: cell surface receptor linked signal transduction; chromosome organization and biogenesis; immune response; metaphase plate congression; mitosis; signal transduction